中文摘要：

[目的]对比分析复合软骨细胞的海藻酸钠凝胶球在体内外的降解特点。[方法]取2个月龄新西兰兔5只,酶解消化获取膝关节软骨细胞,与海藻酸钠混合制成细胞密度为5.8×10^6/ml的混悬液,制成体积为25μl软骨细胞-海藻酸钠凝胶球,在6孔板中进行体外培养对照;体内组取4个月龄新西兰兔36只,双侧股骨滑车行直径4 mm的全层软骨缺损,并将软骨细胞-海藻酸钠凝胶球置入缺损处并经MRI评估缺损模型。分别于1、2、4、6周,通过倒置显微镜观察体外对照海藻酸钠降解情况;通过MRI、大体显微镜和番红O染色观察体内组中海藻酸钠凝胶球体内降解过程。[结果]大体显微观察体外对照组中1周海藻酸钠凝胶球边界清楚,4周凝胶球边界模糊不清,6周藻酸钠边界消失且成糊状。体内组中Roberts MRI评价修复效果显示4周（2.25±0.500）与1周（1.00±0.817）相比差异有统计学意义（t=2.611 P=0.0401）,与2周（1.25±0.500）相比差异有统计学意义（t=2.828,P=0.0300）,与6周（2.75±0.500）相比差异无统计学意义（t=1.416,P=0.2070）;大体观察1周时凝胶球开始液化,4周时海藻酸钠完全消失,且随着凝胶球的降解,缺损以组织修复;番红O染色示实验组修复1、2、4、6周后分别表现为无修复、部分纤维修复、大部分修复、完全纤维修复。[结论]与体外对比,复合软骨细胞的海藻酸钠凝胶球在兔膝关节内生物相容性较好,降解时间明显缩短,大约4周吸收完全,可以为临床应用提供理论依据。

英文摘要：

[Objective]To compare the degradation of sodium alginate beads combined with chondrocytes in vivo and in vitro. [Method]Chondrocytes from the both knee joints of 2- month- old New Zealand white rabbits were digested using collagenase enzyme. The chondrocytes were mixed with sodium alginate in a cell density of 5. 6 × 10^6. The mixture was gelled in a shape of sphere in CaC l2 solution and the diameter of sphere was 3mm. The beads of alginate combined with chondrocytes were cultured in three- dimensional systems cell culture plate（ control group）. Twelve 4- month- old New Zealand white rabbits were chosen,and full- thickness articular defect with a diameter 4 mm were produced in the femoral condyle fossa of both knee joints. Alginate beads combined chondrocytes cultured for two days in vitro was implanted into the articular defects. The defects＇ homogeneity was assessed by MRI（ experimental group）. Inverted microscope was performed to analyze the degradation in both groups at the end of 1,2,4 and 6 weeks,respectively. While the repair effect was deceted by MRI and safranin＇ O histological only in experiment group at 1,2,4 and 6weeks,respectively. [Result]The data from microscope,at the end of 1,2,4 and 6 weeks,respectively,showed that the boundary of sodium alginate spheres in the control group were clear,transparent,blurring and disappeared,and that the ball in the experimental group were starting liquefaction,obvious liquefaction,disappear and tissue repair completely. The result from Roberts MRI showed that the score of repair effect at the end of 4 weeks（ 2. 25 ± 0. 500） was signif-icant higher than 1 week（ 1. 00 ± 0. 817）（ P〈0. 05）. The similar result was found between 4 weeks（ 2. 25 ± 0. 500）and 2 weeks（ 1. 25 ± 0. 500）（ P〈0. 05）. However,there was no difference between 4 weeks（ 2. 25 ± 0. 500） and 6weeks（ 2. 75 ± 0. 500）（ P〉0. 05）. Safranin ＇O staining showed that repair effect in the experimental group displayed non repair,part of the fiber rep