中文摘要：

目的：研究组蛋白伴侣分子SSRP1对DNA损伤的应答情况。方法：从HeLa细胞扩增SSRP1 cDNA,并克隆到EGFP载体上。EGFP-SSRP1在细胞内表达,用405 nm激光刺激诱导DNA损伤,随后观察EGFP-SSRP1的聚集情况。结果：经酶切和测序鉴定,我们成功构建了EGFP-SSRP1表达载体。EGFP-SSRP1在405 nm激光刺激后迅速聚集到激光照射位点。结论：我们的结果直接证实了SSRP1快速参与DNA损伤应答。

英文摘要：

Objective： To investigate the function of histone shaperone SSRP1 in response to DNA damage.Methods： SSRP1 gene was amplified from HeLa cells,cells plated in glass-bottomed and cloned to EGFP vector.EGFP-SSRP1 was transiently transfected into HeLa dishes.Cells expressed EGFP-SSRP1 was micro-irradiated with a 405 nm pulse laser along a user-defined path.The response of SSRP1 to DNA damage was measured by a confocal scanning laser microscopy system.Results： The EGFP-SSRP1 vector was identified and confirmed by enzyme digestion and sequencing.SSRP1 protein was recruited to DNA damage sites induced by 405 nm laser.Conclusion： SSRP1 is associated with DNA damage response.