中文摘要：

目的：制备GPR48的单克隆抗体，并且初步评估其在体外对子宫颈癌细胞侵袭转移的抑制作用。方法：纯化的GPR48特异性片段作为抗原，细胞融合法制备GPR48单克隆抗体，蛋白免疫印迹法检测单克隆抗体的特异性，用体外侵袭性实验检测该单抗抑制子宫颈癌细胞Hela侵袭转移的作用。结果：制备了效价高、特异性好的GPR48单克隆抗体。当加入10nmol／L浓度的单克隆抗体时，对照组和实验组的穿膜细胞数分别为280±18、73±11；而当加入单抗浓度为50nmol／L时，对照组和实验组的穿膜细胞数分别为267±30、14±3。结论：G PR48单克隆抗体在体外能够显著抑制子宫颈癌细胞的侵袭转移。

英文摘要：

Objective ：To obtain mouse anti -human GPR48 monoclonal antibodies and to estimate the inhibition effect on invasion and metastasis of cervical neoplasm cells in vitro. Methods ：The monoclonal antibody of GPR48 was produced by cell fusion and identified by Western blotting. Then the inhibition effect on cancer cells invasion and me- tastasis was measured by Matrigel invasion assay in vitro. Results ：The monoclonal antibody of GPR48 was prepared. When we added 10 nmol/L IgG or GPR48 antibody into the migration assay, the number of migrated ceils was （280 ＋ 18） vs （73 ± 11）. When we added 50 nmol/L antibody,the number of migrated cells was （267 ±30） vs （14 ± 3）. Condusion：GPR48 mAb showed the high specificity to GPR48 with strong affnity and efficient suppression on invasion and metastasis of Hela cells in vitro.