中文摘要：

目的：制备G蛋白偶联受体48（G protein coupled receptor48，GPR48）单克隆抗体，并研究其在结直肠癌中的表达。方法：采用杂交瘤技术制备单克隆抗体，Western印迹法和FCM检测单克隆抗体的特异性，免疫组织化学法检测GPR48在40例结直肠癌中的表达。结果：制备获得GPR48的特异性单克隆抗体；GPR48在正常结直肠黏膜组织中无表达；淋巴结转移者GPR48阳性率（85．7％）明显高于无淋巴结转移者（34．6％）（P〈0．05）；高分化者的GPR48表达阳性率（22．2％）明显低于中、低分化者的77．3％（P〈0．05）；GPR48的表达-9患者的性别和肿瘤大小均无关。结论：GPR48抗体是1种特异性抗体，可将其作为一项新的预测肿瘤分化程度和淋巴结转移的指标。

英文摘要：

Objective ：To prepare the monoclonal antibody of G protein coupled receptor 48 （GPR48） and investigate the expression in colorectal cancers. Methods： The monoclonal antibody of GPR48 was produced by cell fusion technology and identified by Western blotting and flow cytometry （FCM）. The expression of GPR48 was studied in colorectal cancer by using the immunohistochemical method. Results： The specific monoclonal antibody of GPR48 was prepared. No expression of GPR48 was detected in normal colorectal tissues. The positive rate of GPR48 was significantly higher in colorectal cancer patients with lymph node metastasis than that in patients without lymph node metastasis （ 85.7% vs 34.6% , P 〈 0.05 ） ; it was significantly lower in well-differentiated colorectal cancer than that in moderately- and poorly-differentiated colorectal cancer （22.2% vs 77.3%, P 〈 0.05 ）. GPR48 expression had no correlation with the sex and tumor size of patients. Conclusion： The monoclonal antibody of GPR48 is a specific antibody. It can be used as a new index for predicting tumor differentiation and lymph node metastasis.