中文摘要：

从青海省化隆县发病刺儿菜叶片上分离获得菌株HL-1,其发酵滤液对供试阔叶杂草猪殃殃、藜、冬葵、酸模叶蓼的发病率分别为98.10%、90.20%、56.67%和66.67%,病情指数为98.01、88.33、56.71和47.46;鲜质量防效达到69.31%、71.83%、65.54%和56.10%,表现出较好的除草活性。作物安全性试验结果显示,其发酵滤液对小麦、蚕豆安全,对油菜、豌豆和青稞有轻微影响。对该菌株的培养特性和16S r DNA基因序列进行分析,确定其为内生真菌。内生真菌菌株HL-1可以在以猪殃殃和藜为优势杂草的春小麦和蚕豆田中作为微生物源除草剂使用。

英文摘要：

Strain HL-1 isolated from diseased Cirsium setosum ( Willd. ) MB. leaves in Hualong, Qinghai province, was evaluated for its herbicidal activity to target weeds and its safety to crops. The disease incidences of Galium aparine L. , Chenopodium album L. , Malva crispa L. , and Polygonum lapathifolium L. applied with HL-1 filtrate were 98. 10%, 90. 20%, 56. 67%, and 66. 67%, the disease indexes were 98. 01, 88. 33, 56. 71, and 47. 46, and the fresh weight re-ductions were 69. 31%, 71. 83%, 65. 54%, 56. 10%, respectively. The filtrate was safe to wheat and broad bean and slightly pathogenic to rapeseed, pea and highland barly. The strain was identified as fungal endophyte by cultural character-istic and 16SrDNA sequencing. These results indicated that fungal endophyte HL-1 could be a potential microbial herbicide for the control of G. aparine L. and C. album L. dominating in wheat and broad bean fields.