中文摘要：

为了探索研究用于潜艇舱室大气环境中致病微生物的在线检测技术理论，结合目前国际先进的基因芯片技术，设计利用肽核酸作为探针来进行在线检测；理论证明，肽核酸探针与DNA探针相比，其杂交的稳定性和特异性增加，且能在低盐浓度下进行杂交，因此它能够大大提高微生物学检测和医疗诊断的效率和灵敏度。PNA独特的生化属性已逐渐为世人所瞩目，PNA探针技术也得到了迅速发展，尤其是其在微生物检测领域中的应用，在结合潜艇舱室密闭环境的主要特点和舱室内致病微生物的来源和危害的基础上，发展以肽核酸为探针的基因芯片技术。

英文摘要：

On-line detection technology used in the detection of pathogenic microorganisms in submarine cabin atmospheric environment was studied. Peptide nucleic acid probes were designed with the current international advanced gene chip technology for detecting online. It was showed that PNA probes, compared with traditional DNA probes, have higher hybrid-stabilization, better specificity, and it can hybridize target substance under low salt ion concentration. These advances in molecular biotechnology have greatly improved the sensitivity and the efficiency in microbiology investigations and diagnosis. PNA＇s unique physicochemical properties have gradually become a new focus of the world and PNA probes also have developed rapidly. PNA probes＇applications in the field of microbial detection were further discussed. On the base of the major characteristics of submarine cabin and the resource and harm of incurred disease microbe, it was suggested that the development of gene chip used of PNA probe is important.