中文摘要：

目的探讨白花蛇舌草对人肝癌HepG2细胞裸鼠皮下移植瘤组织磷酸酰肌醇3激酶（P13K）、总蛋白激酶B（Akt）和磷酸化Akt（p-Akt）蛋白表达的影响。方法建立肝癌裸鼠皮下移植瘤模型，随机分为药物组（6g·kg^-1·d^-1白花蛇舌草灌胃）和模型组（同等体积生理盐水灌胃），连续给药4周后处死，观察抑瘤率；免疫组织化学染色检测肿瘤组织P13K、总Akt和p-Akt蛋白的表达。结果与模型组比较，药物组瘤体质量明显下降（t=4．270，P=0．006），抑瘤率达61．29％；P13K蛋白表达灰度值从82．00±2．72升高至118．67±8．44（t=0．209，P=0．049）；Akt蛋白表达灰度值从72．37±2．89升高至105．19±4．718（t=2．050，P〈0．001），p-Akt蛋白表达灰度值从87．99±4．16升高至96．34±5．72（t=4．123，P=0．002），提示P13K、Akt和p-Akt的蛋白表达水平均较模型组明显减低。结论白花蛇舌草通过可调控P13K／Akt信号通路的相关蛋白抑制肿瘤血管新生。

英文摘要：

Objective To evaluate the effect of hedyotis diffusa willd on phosphoinositide 3 kinase （ PI3K）, pro- tein kinase B （Akt） protein and phosphorylated Akt （p-Akt） protein expressions in nude mice with hepatic carci- noma transplanted subcutaneously. Methods Nude mice xenografts were established. The mice were randomly divid- ed into 2 groups： the hedyotis diffusa willd group and the vehicle group, which received hedyotis diffusa willd （6 g · kg ^-1· d^-1, intragastric administration ） and normal saline （ intragastric administration ）, respectively. After 4 weeks of treatment,the tumors were harvested and weighed. The levels of PI3K, Akt and p-Akt proteins were ob- served with immunohistochemical staining. Results The tumor weight of the hedyotis diffusa willd group were high- er than that of the vehicle group （t = 4.270, P = 0. 006）. The gray levels of PI3K, Akt and p-Akt in the hedyotis dif- fusa willd group （118.67 ± 8.44,105.19 ± 4. 718,96.34 ± 5.72） were also higher than those in the vehicle group （82.00±2.72,72.37±2.89,87.99 ±4. 16） （t=0.209,P=0.049; t=2.050,P〈0.001; t=4.123,P= 0. 002）. The expressions of PI3K, Akt and p-Akt proteins were suppressed significantly by hedyotis diffusa willd. Conclusion Hedyotis diffusa willd could inhibit angiogenesis in hepatocellular carcinoma via regulating the PI3K/Akt pathway.