中文摘要：

长春花Catharanthus roseus（L.）G.Don为夹竹桃科（Apocynaceae）长春花属（Catharanthus）植物,具有解毒抗癌、清热平肝等功效。为进一步研究其生物碱类活性成分,对其地上部分95%乙醇提取物采用硅胶正相柱色谱及制备高效液相色谱进行了分离纯化,共得到6个单吲哚类生物碱。根据理化性质和波谱数据鉴定了它们的化学结构,分别为vindolinine B（1）、洛柯碱（2）、荷哈默辛碱（3）、文多尼定碱（4）、文多灵（5）和狗牙花定碱（6）。其中化合物1为新化合物,命名为vindolinine B。

英文摘要：

Catharanthus roseus （L.） G. Don is a plant of the Catharanthus genus of Apocynaceae which has been reported to have therapeutic effects of detoxication and anticancer. In order to further study the alkaloid constituents of C. roseus,the aerial parts of the plant were extracted with 95% EtOH,and then treated with 2% H2SO4 and NH3.H2O to obtain total alkaloids. The total alkaloids were separated and purified by column chromatography over silica gel and prepared by high performance liquid chromatography （HPLC）. Their structures were elucidated on the basis of physicochemical properties and spectral data. A new alkaloid together with five known compounds were isolated and identified as vindolinine B （1）,lochnericine （2）,horhammericine （3）,vindorosine （4）,vindoline （5）,and coronaridine （6）. Compound 1 is a new compound and named as vindolinine B.