中文摘要：

本研究采用液相色谱-质谱分离鉴定技术,比较不同直径卵泡液（直径〈2mm、直径2～4mm、直径〉4mm）的蛋白质组分差异,收集显著差异的馏分,还原烷基化处理后酶解成肽段,再经反相色谱二次分离后质谱鉴定。结果表明,在优势卵泡中（直径〉4mm）存在显著高表达的蛋白质峰,质谱鉴定结果为转铁蛋白（transferrin,TRF）。转铁蛋白的功能和表达的变化提示,该蛋白质的高表达可作为潜在排卵的标志物之一。同时,本研究采用的LC-MS技术将为卵泡液大规模蛋白质组分离和表达谱构建提供参考。

英文摘要：

The proteins change in follicular fluid play an important role during oocyte maturation and ovulation.In this study,proteins from differential diameter follicular fluid（Φ2 mm,Φ 2 to 4 mm,and Φ4 mm） were compared using liquid chromatography and mass spectrometry（LC-MS）,The differential fraction was collected and further treated including reduction,alkylation and enzyme digestion,the peptides were identified after second time liquid chromatography separation.The results indicated thatthere was a remarkable protein peak in dominant follicular fluid,the protein was transferrin（TRF） in this peak.The function and expression of TRF suggested that TRF could be an marker of potential ovulation.At the same time,LC-MS technique make the base of buffalo follicular fluid separation and proteome profile construction.