中文摘要：

为检测卵泡内小肽的表达,采用基质辅助激光解离串联飞行时间质谱（MALDI-TOF/TOF MS）建立了成熟卵泡液的多肽表达模型,比较小卵泡（直径〈4mm）、中等卵泡（直径4～8mm）和成熟卵泡（直径〉8mm）的多肽表达变化。结果表明,3种形态的卵泡液存在12个显著差异多肽,其中在成熟卵泡内上调6个,下调有5个;在中等卵泡内上调4个,下调5个。其中分子质量约为1746u的多肽在成熟卵泡中特异性表达,可作为卵泡成熟的潜在生物标志物之一。

英文摘要：

In order to detect the peptides expression profile in follicles,the matrix-assisted laser desorption ionization mass spectrometry was used to establish a model of peptide expression profile and compare expression changes of peptides from small follicles（（φ〈4 mm）,medium follicules（4 to 8 mm） and mature follicles（φ〉8 mm）.The results indicated that there were 12 peptides to express significantly different among three shapes of follicles.6 peptides increased and 5 peptides decreased in mature follicles.The 1746 u of peptide expressed specifically in mature follicles,which might be used as the one of the underlying biomarkers for follicles maturation.