中文摘要：

目的：建立丹参酚酸B的反相高效液相色谱分析方法，并对其在大鼠体内的药代动力学行为特性进行全面的分析研究。方法：生物样品采用液-液萃取方法，以Hypersil C18 ODS色谱柱（200mm×4．6mm，5μm），柱温40℃，流动相：乙腈：水=20：80（含o．25mol／L乙酸胺），用磷酸调pH至4．0，流速：1．0mL／min；紫外检测波长：328nm。结果：鼠尾静脉给予丹参酚酸B1．6、3．2、6．4mg／kg后，结果显示鼠a相半衰期为（3．1±0．1）min，8相半衰期为（31．5±3．2）min。大鼠尾静脉给予丹酚酸B后，组织中浓度依次为（高-低）：心、肝、肺、小肠、肾脏、脾、胃、卵巢和脑组织。丹参酚酸B在粪便和尿中24h及胆汁中2h的累积排泄百分数分别约为1、43％、0．77％及8．03％。丹参酚酸B人血浆蛋白结合率和大鼠血浆蛋白结合率分别为89．2％±1.8％和92．5％±1．5％。结论：本法准确、稳定、灵敏度高，适合生物样品中丹参酚酸B的分析。丹参酚酸B在大鼠体内消除速度快，血浆蛋白结合率较高，胆汁为其主要排泄途径。

英文摘要：

AIM： To establish an HPLC mehod for the analysis of phannacokinetics of salvianolic acid B in rats. METHODS： The biological samples were extracted with acetic ether. The chromatographic conditions were as follows： Hypersil ODS column （200 mm ×4.6 mm, 5μm） was used. The mobile phase was acetonitrile-water （with Ammoniom Acetate 0.25 mol/L） （25 ： 75, v/v） at a flow-rate of 1.0 mL/min, and the detection wave length was set at 328 nm. RESULTS： Salvianolic acid B was injected intravenously at doses of 1.6, 3.2, 6.4 mg/kg. The terminal elimination half-life（ t1.2 ） of a phase and β phase was （3.1±0.1） rain and （31.5＋3.2） rain. The extents of excrement, urine and biliary excretion of salvianolic acid B were 1.43 % ±0.90 %, 0.77 % ± 1.01% and 8.82% ± 4.11%. The tissue concentration of salvianolic acid B was as followed in order： Chcart 〉 Cliver 〉 Clung 〉 Cintestine〉 Ckidney 〉 Cspleen 〉 Cstoolach. The plasma protein binding rate of salvianolic acid B in human plasma and in rat was similar （89.2 % ± 1.8 %, 92.5 % ± 1.5% ）. CONCLUSION： The method is accurate, stable and reliable, and can be used for the investigation of salvianolic acid B in phannacokinetics research. Salvianolic acid B eliminates fast and it shows a high plasma protein binding rate, the mainly excretion way of salvianolic acid B is from biliary.