中文摘要：

Xanthomonas oryzae pv。oryzicola (Xoc ) ，在米饭引起细菌的叶条纹的批评病原体，拥有负责在主人米饭在非主人烟草和致病力触发过分敏感的反应(HR ) 的 hrp 簇，并且被认为是在米饭模型植物的模型病原体之一。这里，我们用二拍子的圆舞集成开发了一个高产量的 mutagenesis 系统由新奇自杀向量 pKMS1 调停了。为功能的分析产生单身者或 hpa1， hpa2， hrcV， hrpE， hpaB，和 hrpF 基因的多基因异种被用来。总共，五个单身者，四加倍，并且二翻三倍 hrp 基因异种被构造。双、三倍的 hrp 基因删除异种在 planta 触发了新奇显型。我们的数据建议 pKMS1 是为在 Xoc 的多重基因的非标记 mutagenesis 的一个有用工具。

英文摘要：

Xanthomonas oryzae pv.oryzicola （Xoc）,the critical pathogen causing bacterial leaf streak in rice,possesses a hrp cluster that is responsible for triggering hypersensitive response （HR） in non-host tobacco and pathogenicity in host rice,and is considered to be one of the model pathogens in the rice model plant.Here,we developed a high-throughput mutagenesis system using a two-step integration mediated by a novel suicide vector pKMS1.It was used to generate single or poly-gene mutants of hpa1,hpa2,hrcV,hrpE,hpaB,and hrpF gene for functional analysis.In total,five single,four double,and two triple hrp gene mutants were constructed.The double and triple hrp gene deletion mutants triggered novel phenotypes in planta.Our data suggest that pKMS1 is a useful tool for non-marker mutagenesis of multiple genes in Xoc.