中文摘要：

可信的证据证明有气味的东西绑定蛋白质(OBP ) 为昆虫嗅感觉被要求并且在越过 sensillum 淋巴把恐水病的有气味的东西搬运到嗅觉的受体(ORs ) 起一个关键作用。在现在的学习，从紫花苜蓿植物臭虫的新奇 OBP (AlinOBP3 ) 基因， Adelphocoris lineolatus，被克隆并且表示。AlinOBP3 的表示模式被 qPCR 评估，它显示 AlinOBP3 主导地在天线被表示。有 9 棉花 volatiles 和 5 性 pheromone 类似物的 AlinOBP3 的有约束力的性质被荧光测量有荧光探查 1-NPN 的竞争有约束力的试金。结果表明 9 棉花 volatiles， Myrcene， -Ocimene 和 -Phellandrene 的能与 AlinOBP3 绑。-Phellandrene 特别与一种高有约束力的亲密关系跳了到 AlinOBP3，与 56.68 mol/L 的一个分离常数。5 性 pheromone 类似物，己基的丁酸盐与 AlinOBP3 有最强壮的有约束力的亲密关系，与象 59.53 mol/L 的一个分离常数。丁基丁酸盐， trans-2-Hexenyl 丁酸盐和乙醇丁酸盐分别地与 AlinOBP3 有中等有约束力的亲密关系，与 227.39， 108.77 和 143.47 mol/L 的分离常数。结果建议那 AlinOBP3 可能是 pheromone 绑定蛋白质(PBP ) 与一为性 pheromones 和植物 volatiles 的感觉双功能。

英文摘要：

Credible evidence shows that odorant binding proteins （OBPs） are required for insect olfaction perception and play a key role in transporting hydrophobic odorants across the sensillum lymph to the olfactory receptors （ORs）. In the present study, a novel OBP （AlinOBP3） gene from the lucerne plant bug, Adelphocoris lineolatus, was cloned and expressed. The expression pattern of AlinOBP3 was evaluated by qPCR, which indicated that AlinOBP3 was dominantly expressed in antennae. The binding properties of AlinOBP3 with 9 cotton volatiles and 5 sex pheromone analogs were measured by fluorescence competitive binding assays with the fluorescence probe 1-NPN. The results revealed that of 9 cotton volatiles, Myrcene, 13-Ocimene and ct-Phellandrene can bind with AlinOBP3. α-Phellandrene especially bound to AlinOBP3 with a high binding affinity, with a dissociation constant of 56.68 μmol/L. Of the 5 sex pheromone analogs, Hexyl butyrate had the strongest binding affinity with AlinOBP3, with a dissociation constant as 59.53 μmol/L. Butyl butyrate, trans-2-Hexenyl butyrate and Ethyl butyrate had medium binding affinities with AlinOBP3, with dissociation constants of 227.39, 108.77 and 143.47 μmol/L, respectively. The results suggest that AlinOBP3 might be a pheromone binding protein （PBP） with a dual-function for the perception of sex pheromones and plant volatiles.