中文摘要：

通过染色体步移方法从银杏（Ginkgo biloba L.）基因组中克隆到查尔酮合成酶基因（CHS）翻译起始位点上游1711bp的启动子序列。生物信息学分析表明,该启动子片段中存在多个顺式作用元件,包括紫外/蓝光响应单元、植物激素响应单元、真菌诱导元件、MYB结合位点、TATA-box和CAAT-box等。亚克隆了CHS转录起始位点上游1402bp序列,将其与GUS基因构建融合表达载体pBI121＋CHSP,以pBI121-35S作为负对照,通过农杆菌（LBA4404）介导法分别转入烟草。结果表明,银杏CHS启动子序列能驱动GUS基因在烟草中的表达,表达具有组织特异性。GbCHSP的功能研究将有助于揭示银杏叶黄酮的积累与GbCHS基因表达的分子机理。

英文摘要：

The regulative sequence（1 711 bp）of chalcone synthase gene promoter（CHSP）from Ginkgo biloba L. was cloned by genomic walking. In silico analysis suggested that the sequence contained several typical cis-acting elements,including UV/blue light responsive elements,phytohormone responsive elements,fungal elicitor responsive elements,MYB binding site,TATA-box and CAAT-box. A 1 402 bp promoter sequence upstream 5′ of translation start site of GbCHS was cloned and designated as GbCHSP, respectively. pBI121 ＋ CHSP and pBI121-35S were constructed and transformed into tobaccos by LBA4404. These results showed that pBI121 and pBI121 ＋ CHSP both could drive the transient expression of GUS in tobaccos and pBI121 ＋ CHSP expressed differentially in root,stem and leaf tissues of tobacco. These results will be help to understand the transcriptional regulatory mechanism on GbCHS expression and flavonoids accumulation.