中文摘要：

目的：构建能够稳定克隆、表达RIZ1基因中PR结构域的真核表达载体。方法：首先用RT-PCR方法从人食管组织中提取mRNA,逆转录为cDNA,利用cDNA模版扩增出PR结构域,连入T载体。然后提取PR结构域及pcDNA3.1（＋）质粒,酶切后连接,转入Trans-T感受态细胞中。结果：提取pcDNA3.1/PR结构域质粒,琼脂糖凝胶电泳,可见大于5 000 bp的目的条带,与预测结果一致,测序结果正确。结论：构建PR结构域真核表达载体成功,为进一步实验研究PR结构域在食管癌细胞中的表达与功能奠定了基础。

英文摘要：

Objective： To construct human PR domain eukaryotic expression vectors in order to research the function of RIZ1. Meth- ods：At first, mRNA was extracted from human esophageal cancer tissue by RT-PCR, and transcribed reversely to cDNA, PR domain am- plifying from DNA template linked to T vector. Second, the plasmid of PR domain and pcDNA3.1 （＋） were extracted, cut with enzyme and linked, and then, transfered to Transl-T1 phage resistant competent ceils. Results： More than 5 000 bp purpose strap of the pcDNA3.1 （＋）/PR domain plasmid was found by the method of agarose gel electrophoresis. Conclusion： PR domain eukaryotic expression vectors is constructed successfully. This is the fundament for the further experimental study of expression and function of the PR domain in esophageal cancer cells.