中文摘要：

目的观察依硫磷酸对人慢性髓细胞白血病K562细胞系增殖的抑制作用。方法将依硫磷酸（粉末）以1640培养液稀释成0.2mg/ml、0.5mg/ml、0.75mg/ml、1.0mg/ml、1.6mg/ml、3.2mg/ml共6个浓度组,分别处理增殖期人慢性髓细胞白血病K562细胞,镜下观察不同时间点各浓度组细胞生长情况,应用CCK-8法检测细胞增殖能力,绘制生长曲线,计算抑制率和半数抑制浓度（IC50）。结果依硫磷酸对K562细胞增殖具有抑制作用,细胞倍增时间为48h,并且具有时间和剂量依赖性。在给药48h〉0.75mg/ml浓度的依硫磷酸对K562细胞增殖具有抑制作用（P〈0.05）,而3.2mg/ml浓度的依硫磷酸与K562细胞培养72h后对其增殖抑制作用最强,抑制率为91%。结论依硫磷酸对K562细胞增殖具有抑制作用,为临床应用依硫磷酸治疗慢性髓细胞白血病提供了实验基础。

英文摘要：

Objective To observe the effect of amifostine on proliferation of K562 cells.Methods Amifostine was proportionally diluted to 0.2mg/ml,0.5mg/ml,0.75mg/ml,1.0mg/ml,1.6mg/ml,and 3.2mg/ml groups.K562 cells were incubated with different concentrations of amifostine and cell growth status was chronologically observed under a microscope.Cell proliferation was detected by CCK-8 assay and cell growth curve was plotted for calculating inhibition ratio.Results Amifostine could remarkably inhibit the proliferation of K562 cells with a cell doubling time of 48h.Amifostine inhibited the proliferation of K562 cells in a time-and dose-dependent manner.The proliferation of K562 cells was significantly inhibited 48h after treatment with amifostine at the concentration of 0.75mg/ml or higher（P0.05）.The greatest inhibitory rate was 91% in 3.2mg/ml amifostine group with numerous cells died.Conclusion Amifostine can inhibit the proliferation of K562 cells,which provides an experimental basis for the treatment of hematological toxicity which is correlated with chemotherapy for chronic myeloid leukemia with amifostine.