中文摘要：

为分析LRP16基因启动子区顺式调控元件，为深入研究LRP16基因的表达调控机制奠定基础。首先在NCBI的人类基因组数据库中截取并下载LRP16基因转录起始位点5’侧翼区2．6kb的基因组序列，设计PCR引物，从健康外周血单个核细胞中扩增LRP16基因的启动子分子；然后构建包含长度为2．6kb的LRP16基因启动子分子的pG13-LRP16启动子荧光素酶报道重组子，转染入Hela细胞后检测荧光素酶活性；最后利用Genomatix MatInspector Release professional 5．3、RSA—tools和TESS3个启动子顺式调控元件数据库对LRP16基因启动子进行分析。结果表明LRP16基因启动子DNA序列具有真核启动子活性，该区域既有通用启动子结构，又具有与肿瘤发生、细胞周期调控和急性反应期蛋白有关的顺式调控元件，包括：Spl、T—Ag、ZF5、CAC盒、PU．1、c—Ets、XPF-1、IL-6受体、雌激素受体和视黄醇受体。

英文摘要：

To analyze the cis elements in LRP16 gene promoter region and to explore the possible regulation mechanism of LRP16 gene expression. Firstly, a 2.6kb DNA sequence of LRP16 5＇ -end was obtained from NCBI by BLAST software. The 2.7kb long target sequence from a healthy blood donor DNA sample were amplified by PCR amplification, then the product were identified by DNA sequencing. After that the verified sequence was inserted into pGL3 - Basic luciferase vector, then the relative luciferase activity was detected with a Luminometer. At last, the 2.6Kb DNA sequence upstream the LRP16 gene was analyzed online with Genomatix MatInspector Release professional 5.3, RSA- tools and TESS. The results shouwed LRP16 gene promoter has both general and special cis-acting elements relating to cell cycle, hematopoiesis, cell proliferation, carcinogenesis and acute reacting process, such as Spl, T - Ag, ZF5, CAC box, PU. 1, c - Ets, XPF - 1, IL - 6RE, ER and RAR.