中文摘要：

目的：观察促红细胞生成素（EPO）对帕金森病（PD）模型大鼠的保护作用并探讨其机制。方法实验大鼠分为3组，①PD组：6-羟基多巴（6-OHDA）定向注射至右侧前脑内侧束建立PD模型；②EPO组：在模型制备前持续1周给予腹腔注射EPO10000U·kg-1·d-1；③假手术组：大鼠脑内立体定向注射生理盐水。采用ELISA法检测大鼠脑脊液EPO含量变化，术后3周评估大鼠行为学改变，免疫组化学、免疫荧光及蛋白印迹法分别检测酪氨酸羟化酶（TH）、EPO受体（EPO-R）及丝氨酸/苏氨酸激酶（Akt）磷酸化（Ser473）的表达。结果①EPO组脑脊液内EPO含量[（416.59±9.34）μU·L-1]增加，与假手术组[（2.20±1.92）μU·L-1]比较，差异有统计学意义（P﹤0.05）；②EPO组的行为学改善，黑质多巴胺能神经元的丢失减少，黑质EPO-R的表达增加，与PD组及假手术组比较，差异有统计学意义（P﹤0.05）；③EPO组黑质Akt磷酸化（Ser473）的表达明显增加，与PD组及假手术组比较，差异有统计学意义（P﹤0.05）。结论 EPO腹腔注射可以改善PD大鼠的运动症状，与EPO增加PD大鼠的磷酸化Akt（Ser473）表达可能有关。

英文摘要：

Aim To investigate the neuroprotection of erythropoietin in a rat model of Parkinson’s disease （PD） and explore the mechanisms underlying these effects. Methods The rats were divided into three groups. The rats model of PD were induced by stereotactic injection of 6-OHDA.EPO （10 000 U·kg-1·d-1） was continuously administered intraperitoneal injection for a week to the rats before 6-OHDA injection. The sham operation group stereotactic injected of saline. Neurobehavior in rats were measured in 3 weeks after the treatments.Survival of substantia nigra （SN） dopaminergic neurons, tyrosine hydroxylase （TH） levels and EPO-R levels and phosphoprotein of serine-threonine kinase（Akt） at serine 473 levels were determined by immunohistochemistry and Western blot respectively. Results There was a significant increase in EPO levels in the CSF after EPO administered. Neurobehavior was signiifcantly improved in the rats that were pretreated with EPO compared with PD rats. The dopaminergic neurons in SN were also increased in the EPO pretreated rats when compared with the PD rats. Western blot analysis showed that EPO-R and phosphorylated Akt Ser473 in SN were increased significantly compared with PD and sham rats （P﹤0.05）. Conclusion These findings suggested that EPO exerted neuroprotective effects in reversing behavioral deifcits may be associated with PD through the Akt pathway.