中文摘要：

目的观察关节腔内注射臭氧对胶原诱导性关节炎（CIA）大鼠血清中TNF-α、IL-6、IL-17A及血管内皮生长因子（VEGF）的影响，探讨臭氧治疗RA的作用机制。方法将32只体质量及鼠龄相当的Wistar大鼠采用随机数字表法，随机分为正常对照组（对照组）、CIA模型组（CIA组）、臭氧组、甲氨蝶呤组。除正常对照组外，采用注射弗氏完全佐剂＋牛Ⅱ型胶原蛋白的方法建立CIA大鼠模型。造模后14d，臭氧组双踝关节内注射浓度为40μg／ml的臭氧各1ml，每周1次，共注射3周。甲氨蝶呤组0．9mg／kg，每周1次，共3周。分别测量各组大鼠双后肢足肿胀度。治疗结束后1周，用流式多因子检测技术测定各组大鼠血清中TNF-α、IL-6、IL-17A及VEGF的水平。采用单因素方差分析或Kmskl—Wallis秩和检验进行统计分析。结果治疗结束后，臭氧组大鼠足肿胀度减小，与模型组相比差异有统计学意义[（4．21±0．14）ml与（9．12±0．17）ml，t=8．43，P〈0．05].与CIA模型组相比，臭氧组血清TNF-α、IL-6及VEGF浓度明显降低，差异有统计学意义[91．55（86．55，98．53）pg／ml与14．45（12．55，16．15）pg／ml，X2=6．216，P=0．002；145．08（37．44，362．82）pg／ml与5．84（5．47，15．93）pg／ml，X2=13．136，P=0．004；51．56（46．09，74．10）pg／ml与36．22（32．18，41．69）pg／ml，X2=3．732，P=0．002]，臭氧组血清TNF-α、IL-6、VEGF浓度与甲氨蝶呤组相比，差异无统计学意义[14．45（12．55，16．15）pg／ml与（12．45（11．80，15．60）pg／ml，r=0．243，P〉0．05；5．84（5．47，15．93）pg／ml与7．86（5．25，15．25）pg／ml，r=0．058，P〉0．05；36．22（32．18，41．69）pg／ml与40．17（35．47，50．73）pg／ml，X2=0．516，P〉0．05].臭氧组与对照组、CIA组、甲氨蝶呤组3组间血清IL-17A水平相当，差异无统计学意义（F=1．827，P=-0．165）。结论?

英文摘要：

Objective To observe the effects of intra-articular ozone injection on the secretion of tumor necrosis factor （TNF）-α, Interleukin （IL）-6, IL-17A, and vascular endothelial growth factor （VEGF） in the serum of rats with collagen-induced arthritis （CIA） and explore the therapeutic mechanism of ozone in RA treatment. Methods Thrity-two Wistar rats were randomized into 4 groups, including the ozone groups that receivedintra-articularinjection of 40 μg/ml ozone （03 group）, a blank control group （normal group）, a methotrexate （MTX） group （MTX group） and a collagen-induced arthritismodel （CIA group）. All the rats, except for those in the blank control group, were subjected to hypodermic injection of bovine collagen Ⅱ and complete Freund＇s adjuvant to induce CIA. Ozone treatment was administered once weekly for 3 weeks starting at 14 days after the model were established. MTX group were treated with methotrexate 0.9 mg/kg, once a week, a total of three weeks. The swelling degree of the foot were observed, and the serum contents of TNF-α, IL-6, IL-17A and VEGF were detected. One-way analysis of variance or Kruskal-Wallis test was used to evaluate the experimental data. Results At the end of treatment, the degree of foot swelling was reduced significantly in rats with 03 group compared with that in the CIA group [（4.21±0.14） ml vs （9.12±0.17） ml, t=8.43, P=0.023]. The serum concentration of TNF-α, IL-6 and VEGF showed significant difference between the CIA group and the 03 group[91.55（86.55, 98.53） pg/ml vs 14.45（12.55, 16.15） pg/ml, X2=6.216, P=0.002; 145.08（37.44± 362.82） pg/ml vs 5.84（5.47,15.93） pg/ml,X2=13.136, P=0.004; 51.56（46.09, 74.10） pg/ml vs 36.22（32.18, 41.69） pg/ml, X2=3.732, P=0.002]. There was no statistieally significant difference between the 03 group and MTX group [14.45（12.55, 16.15） pg/ml vs [12.45（11.80, 15.60） pg/ml, X2=0.243, P〉0.05; 5.84（5.47, 15.93） pg/ml vs 7.86（5.25, 15.23） pg/ml,X2=0.058, P〉0.05;