中文摘要：

目的比较RA患者与健康人骨髓间充质干细胞（BMSCs）的增殖能力及端粒酶反转录酶（hTERT）的表达，探讨RA患者BMSCs是否存在缺陷或变异，能否用于自体移植。方法取8例RA患者与8名健康志愿者5ml骨髓，分离、培养、传代，用流式细胞术对其表面标记进行鉴定，四甲基偶氮唑蓝（MTr）法测定P3、P6代BMSCs生长曲线，计算细胞群体倍增时间；流式细胞术测定细胞周期，计算增殖指数；实时荧光定量PCR测定hTERT的表达。组间数据比较采用t检验。结果RA患者BMSCs在形态学上与健康对照组无差异。经流式细胞仪检测，证明获得细胞为BMSCs。健康对照组与RA组P3代细胞群体倍增时间（4．7±0．3）d，（4．8±0.3）d，2组差异无统计学意义（P〉0．05）；P6代健康对照组细胞群体倍增时间（5．7±0．4）d，RA组（6．3±0．3）d，RA组BMSCs群体倍增时间长于健康对照组，差异有统计学意义（P〈0．001）。健康对照组P3代BMSCs增殖指数（17．9±2．1）％，RA组（16．9±2．1）％，2组差异无统计学意义（P〉0．05）；P6代健康对照组BMSCs增殖指数（10．7±1．8）％，RA组（6．2±2．2）％，RA组增殖指数小于对照组，差异有统计学意义（P〈0．01）。健康对照组P6代MSCshTERT表达量为（10．6±1．5），RA组为（3．1±1．0），RA患者hTERT表达水平较正常对照组低，差异有统计学意义（P〈0．01）。结论2组人群P3代BMSCs细胞增殖能力的差异无统计学意义，P6代时RA患者BMSCs增殖能力滞后于正常组，推断RA患者BMSCs较健康人衰老速度加快；hTERT测定显示RA组BMSCs的hTERT表达量低于正常对照组，说明这种提前老化可能与hTERT表达过低有关。

英文摘要：

Objective By comparing the proliferation and the human telomerase reverse transcriptase expression of bone marrow mesenchymal stem cells （BMSCs） from rheumatoid arthritis （RA） patients and normal human to explore the existence of BMSCs defects or variation in RA patients, and whether these cells could be used in autologous transplantation. Methods BMSCs were obtained from bone marrow of eight RA patients and healthy volunteers. The cells were isolated andcuhured by gradient centrifugation and adherence cultivation,and the surface markers of BMSCs were identitfied by flow cytometertry. Firstly, BMSCs reproductive activity of the two groups were compared： the auxodrome and cell cycle of BMSCs were measured respectively, then the generation doubling time of cell colony and proliferation index were calculated in order to see whether there was a difference of the proliferation capability between the two groups. Furthermore, the hTERT were measured. Results BMSCs were no significant differences behveen morphology of RA patients and healthy control group. The cells were obtained proof MSCs by flow cytometry. The difference was not statistically significant （P〉0.05）, RA group and the healthy control group P3-generation cell population doubling time （4.7±0.3） d, （4.8±0.3） d. The difference was statistically significant （P〈0.01）, P6 generation of healthy control group cell population has doubling time （5.7±0.4） d, RA group （6.3±0.3） d, buut the group RA BMSCs population doubling time is hmger than the healthy control group. Tile difference was not statistically significant （P〉0.05）, on one hand of the healthy control group P3 BMSCs proliferation index （17.9±2.1）%, on the other hand RA group is （16.9±2.1）%. The difference was statistically significant （P〈0.01）. P6-generation BMSCs proliferation index in healthy control group （10.7±1.8）%, RA group （6.2±2.2）%, RA group proliieration index than the control group. The different was also significarit （P?