中文摘要：

为建立中药活性成分的抗体测定技术平台，将中药活性成分芍药苷与人血清白蛋白偶联成完全抗原（半抗原-蛋白质复合物），并利用基质辅助激光解吸电离-飞行时间-质谱（MALDI-TOF-MS）技术测定了上述人工抗原的基本质谱特征，求得了芍药苷-人血清白蛋白复合物的半抗原数（hapten number）为12．试验结果表明，利用MALDI-TOF-MS方法测定人工合成的半抗原-蛋白质复合物的质谱特征等，具有所需样品量少、结果准确和迅速可靠等优点，为药用植物活性成分的抗体技术研究提供了科学方法和试验依据，对于判断人工抗原的质量具有科学意义．

英文摘要：

In the establishment of monoclonal antibody technology platform, hapten - carrier protein conjugates of a bioactive constituent paeoniflorin （PF） from traditional Chinese herbal medicine and human serum albumin （HSA） were artificially synthesized, and the hapten number of the PF-carrier protein conjugates （PF-HSA） was determined successfully with MALDI-TOF-MS. The results showed the hapten number of the synthesized PF-HSA was 12. This approach would help to determine the quality of artificial antigen, and contributes to antibody preparation of anti - bioactive constituents from traditional Chinese medicines.