中文摘要：

目的研究prosaposin基因对转录活性因子和免疫细胞表面分子表达的影响,分析该基因在各种Toll-like re-ceptor不同途径中的作用,初步探讨prosaposin基因在免疫系统中的功能。方法克隆prosaposin基因并建立prosaposin基因稳定转染的细胞系,通过检测报告基因化学发光和用不同Toll-like receptor配体刺激后比较细胞表面分子的表达水平的方法来探讨不同途径中该基因发挥的作用。结果在TLR3配体PolyI:C刺激条件下,prosaposin基因能够明显促进对NF-κB以及AP-1的活性,而且明显抑制细胞表面分子B7H1和PD1的表达。结论prosaposin基因能够影响TLR3信号途径作用于免疫细胞,调节免疫相关细胞分子,可能在免疫系统方面起作用。

英文摘要：

Objective To determine the effects of prosaposin on the intracellular signal pathways mediated by different kinds of Toll-like receptors(TLRs).Methods Prosaposin gene was cloned and stable prosaposin transfected cell lines were set up;the activity of both NF-κB and AP-1 transcription factors were detected via chemiluminescence method.Flow cytometric analysis was used to investigate the expression of surface molecules in the prosaposin transfected RAW264.7 upon exposure to LPS,PolyI:C,PGN,and BDNA.Results Pr...