中文摘要：

目的克隆并构建人RGS1表达载体,转染U937细胞和树突细胞,进行功能分析。方法通过RT-PCR方法克隆人RGS1基因,将其连接到pcDNA 3.1/V5表达载体。然后以连接的质粒为基础通过报告基因化学发光及ELISA方法对人RGS1基因的进行初步分析。结果经扩增及序列测定表明pcDNA-RGS1质粒构建成功,在U937细胞和树突细胞中可以表达。RGS1对U937细胞转录因子NFκ-B的活性有明显的抑制作用,同时明显上调树突细胞细胞因子IL-6的表达。结论在pcDNA-RGS1质粒成功构建基础上,证明了人RGS1基因具有免疫调节功能,为进一步研究其免疫应答中的调节作用奠定了基础。

英文摘要：

Objective To clone and construct the RGS1 expression plasmid and investigate its expression and function following transfection into U937 and dendritic cells.Methods Human RGS1 was cloned to pcDNA3.1/V5 plasmid,and then the functional potential of human RGS1 in regulating immune responses was investigated via the chemiluminescence and ELISA techniques.Results The plasmid was confirmed by PCR amplification and DNA sequencing,while target gene expression was detected in U937 and dendrite cells.RGS1 could inhi...