中文摘要：

采用UV—Vis光谱法研究了pH=g．28的缓冲溶液中茜素红S（ARS）·Al（Ⅲ）配合物与牛血清蛋白（BSA）的结合反应．研究发现，ARS—Al（Ⅲ）一BSA的最大吸收波长为510nm，比ARS红移82nm，比ARS—BSA红移75nm，比ARS—Al（Ⅲ）配合物红移74nm．ARS—Al（Ⅲ）一BSA三元配合物的结合比为nARS：nA（Ⅲ）：nBSA=6：4：1，物质的量吸光系数ε为2．65×10^4L·mol^-1·cm^-1，ARS—Al（Ⅲ）配合物与BSA作用的条件平衡常数K为8．80×10^8L·mol^-1．ARS—Al（Ⅲ）配合物与BSA之间的作用力主要为配位键和电荷力．

英文摘要：

The interaction of alizarin red S （ARS） -aluminum （ Ⅲ ） complex with albumin bovine scrum （BSA） was investigated by UV-V is spectrophotometric method in acidity buffer solution with pH of 4. 28. It is indicated that ARS-Al（ Ⅲ）-BSA is a red color coordination compound possessing the maximum absorption of 510 nm with red shift of 82 nm, 75 nm and 74 nm compared to ARS, ARS-BSA and ARS-Al（Ⅲ） complex respectively. Its amount absorptivity coefficient is 2. 65 × 10^4 L·mol^-1 · cm^-1, and its combination proportion is defined as nARS ： nAI（Ⅲ） ： nBSA = 6 ： 4 ： 1. Condition equilibrium constant of reaction of ARS-Al（m） with BSA is 8.80 ×10^8 L^-1 mol^-1. The combined action between BSA and ARS-Al（Ⅲ） is the coordinate bond and electrostatic force.