欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
目的研究温石棉及其代用纤维对中国仓鼠肺细胞（V79细胞）p16及p53表达的影响,探讨温石棉及其代用纤维的致癌机制。方法将不同种类（四川新康温石棉、陕西陕南温石棉、玻璃纤维、陶瓷纤维、硅灰石、岩棉）及不同浓度（1-10 mg/L）的温石棉及其代用纤维暴露于V79细胞中,并设阴性对照。采用免疫组化SABC法检测V79细胞p16及p53的表达,并进行比较。结果与阴性对照比较,各染毒V79细胞中的抑癌基因p16和p53表达下调,P均〈0.05;随着温石棉及其代用纤维暴露浓度的增加,V79细胞p16和p53的表达减弱,其OD值也呈下降趋势（P均〈0.05）。结论温石棉及其代用纤维均有不同程度的致癌性;其诱导癌症发生的机制可能与下调抑癌基因p16和p53表达有关。
英文摘要：
Objective To investigated the effects and possible carcinogenic mechanism of chrysotile and its substitute on p16 and p53 expression of V79 hamster cells.Methods Chinese hamster lung cells（ V79 cells） were exposed to the suspension of chrysotile and its substitute [（ Xinkang Sichuan（ A）,chrysotile from Southern Shanxi（ B）,glass fiber（ C）,ceramic fiber（ D）,wollastonite（ E） and rockwool（ F） groups and control group（ G） ]with different powder content（ 1-10 mg /L）.The p16 and p53 expression in V79 cells was tested by immunohistochemistry method,then p16 and p53 statistics were compared.Results Compared with the control group,the antioncogene expression of p16 and p53 was down-regulated in V79 cells of the six experiment groups（ P 〈0.05）.With the increase of exposure concentrations,the expression of p16 and p53 were decreased and OD value of p16 and p53 were decreased（ all P〈 0.05）.Conclusions Chrysotile and its substitute show different levels of carcinogenicity.The carcinogenic mechanism of chrysotile and its substitute may be related to down-regulation of the tumor gene expression of p16 and p53.
同期刊论文项目
同项目期刊论文
期刊信息
位置：