中文摘要：

在我们的以前的研究， Magnaporthe grisea， A1-412，它在 appressorium 形成有缺点，穿入和传染生长的非病原的异种，被 T-DNA insertional 傻瓜获得发育不全。这里，我们报导了相应基因的鉴定和描述。TAIL-PCRtechnique 孤立的 genomic DNA franking T-DNA 的顺序分析证明 T-DNA 被插入到预言的 G proteiny 子单元基因 MGG1 的倡导者区域(为 Magnaporthe grisea G 蛋白质 Gamma 子单元) 。MGG1 被预言与象领域(GGL ) 和 C 终端 CAAX 盒子一样的一个典型 G 蛋白质鲸鱼群妈一起编码蛋白质。MGG1 的 amino-acidsequence 与另外的细丝状的真菌的 G gamma 子单元高度相同。A1-412 的 Furtherphenotypic 调查证明那个外长的营地能导致 appressorium 形成，尽管形成的 appressoria 在形状是反常的并且不能渗透洋葱外皮或米饭叶子。而且，当 A1 -412 与 appropriatemat-ing-type 紧张被穿过时，很少 perithecia 被观察。在 A1-412 的上述显型被基因 MGG1 的再介绍部分补充。我们的结果显示 G 蛋白质鲸鱼群妈子单元 MGG1 可以涉及调整形态发生，交配并且在 M 的致病力。grisea。

英文摘要：

In our previous studies, a nonpathogenic mutant of Magnaporthe grisea, A1-412, which was defective in appressorium formation, penetration and infectious growth, was obtained by T-DNA insertional mutagenesis. Here we reported the identification and characterization of the corresponding gene. Sequence analysis of the genomic DNA franking T-DNA isolated by TAIL-PCR technique showed that T-DNA was inserted into the promoter region of the predicted G protein γ-subunit gene MGG1 （for Magnaporthe gdsea G protein Gamma subunit）. MGG1 is predicted to encode a 93-aa protein with a typical G-protein gamma like domain （GGL） and C-terminal CAAX box. The amino-acid sequence of MGG1 is highly identical to the Gy subunits of other filamentous fungi. Further phenotypic investigation of A1-412 showed that exogenous cAMP could induce appressorium formation, although the formed appressoria were abnormal in shape and unable to penetrate onion epidermis or rice leaves. Moreover, few perithecia were observed when A1-412 was crossed with the appropriate mating-type strain. The above phenotypes in A1-412 were partially complemented by reintroduction of the gene MGGI. Our results indicate that the G-protein gamma subunit MGG1 may be involved in regulating morphogenesis, mating and pathogenicity in M. grisea.