中文摘要：

目的：研究免疫性肝纤维化大鼠肝组织基质金属蛋白酶9/13和基质金属蛋白酶组织抑制因子1/2表达的动态变化及下瘀血汤对其影响,探讨下瘀血汤抗肝纤维化的机制。方法：猪血清腹腔复制大鼠肝纤维化模型,造模8周后ig下瘀血汤的流浸膏,用药4周后和在造模过程中的1、24、、8周动态处死大鼠,获取标本,检测指标。结果：MMP-2/9的高活性和TIMP-1的高表达贯穿于本模型的始终。MMP-9/13蛋白表达在肝纤维化发生发展过程中有先抑制后升高,再次抑制而后升高的反复过程,并受TIMP-1/2表达影响;下瘀血汤可以提高MMP-9活性,促进MMP-9/13而抑制TIMP-1,2表达。结论：该模型有MMP-9/13和TIMP-1/2表达的失衡;下瘀血汤可通过调控MMp-9/13和TIMP-1/2的表达,降解过度沉积的细胞外基质而发挥抗肝纤维化的作用。

英文摘要：

Objective：Changes of protein expression of MMP-9/13 and TIMP1/2 in fibrotic liver tissue induced by porcine serum and effect of Xiayuxue Decoction on it are investigated in this study to explore mechanism of Xiayuxue Decotion in reversing it. Methods：Liver fibrosis in rats was induced by intraperitoneal injection of porcine serum. After 8wk of modeling, rats were given Xiayuxue Decoction for 4 weeks. Rats were sacrificed and sample was obtained for determination at 1 wk, 2 wk, 4 wk, 8 wk and the end of 12 wk of modeling for determination. Results： High activity of MMP-2/9 and high expression TIMP-1 characterizes this liver fibrosis model from beginning to the end. Decreased protein expression of MMP-9/13 in early stage and increased protein expression of MMP-9/13 in early stage and increased protein expression of MMP-9/13 affect by protein expression TIMP-1/2. Xiayuxue Decoction can increase activity of MMP-9, promote expression of MMP-9/13, inhibit expression of TIMP-1/2. Conclusions： Imbalanced MMP-9/13 and TIMP-1/2 expression is seen in this model. The anti-liver fibrosis mechanism of Xiayuxue Decoction is regulating MMP-9/13 and TIMP-1/2 expression to degrade excessive deposition of extracellular matrix.