中文摘要：

[目的]研究免疫性肝纤维化大鼠肝组织基质金属蛋白酶9（MMP-9 mRNA）和基质金属蛋白酶组织抑制因子1（TIMP-1 mRNA）基因表达的动态变化及下瘀血汤对其影响,探讨下瘀血汤抗肝纤维化的机制。[方法]猪血清腹腔注射复制大鼠肝纤维化模型,造模8周后灌胃给予下瘀血汤流浸膏干预治疗,用药4周后和造模过程中的1、2、4、8周动态处死大鼠,获取标本,检测指标。[结果]与正常组相比,模型组1、8、12周MMP-9 mRNA表达明显降低（P〈0.05,〈0.01）,2周MMP-9 mRNA表达显著升高（P〈0.01）,4周表达无明显变化。与模型组12周相比,下瘀血汤组MMP-9 mRNA表达明显升高（P〈0.05）。与正常组相比,1、2、4、8和12周模型组TIMP-1 mRNA表达均有显著升高（P〈0.05,〈0.01））。与12周模型组相比,下瘀血汤组TIMP-1 mRNA表达显著降低（P〈0.01）。[结论]大鼠猪血清免疫性肝纤维化存在着MMP-9 mRNA和TIMP-1 mRNA的动态变化,下瘀血汤可通过促进MMP-9 mRNA表达和抑制TIMP-1 mRNA表达而发挥抗肝纤维化的作用。

英文摘要：

[Objective]To investigate the changes of mRNA expression of matrix metalloproteinases-9（MMP-9）and tissue inhibitor of metalloproteinase-1（TIMP-1）in fibrotic liver tissue induced by porcine serum and effect of Xiayuxue Decoction,so as to highlight anti-fibrotic mechanism of Xiayuxue Decotion.[Methods]Liver fibrosis in rats was induced by intraperitoneal injection of porcine serum.After 8wk of modeling,rats were given Xiayuxue Decoction for 4 weeks.Rats were sacrificed and sample was obtained for determination at 1w,2w,4w,8w and the end of 12 wk of modeling for determination with RT-PCR.[Results]Compared to that in normal group,MMP-9 mRNA expression in model group of 1,4,8 and 12 wk decreased significantly,while the expression in model group of 2wk increased significantly;compared to that in 12wk model group,MMP-9 mRNA expression increased significantly.Compared to that in normal group,TIMP-1 mRNA expression in model group of 1,2,4,8 and 12 wk increased significantly;compared to that in 12wk model group,TIMP-1 mRNA expression decreased significantly.[Conclusion]Dynamic changes of mRNA expression of MMP-9 and TIMP-1 were seen in fibrotic liver tissue induced by porcine serum in rats,and the anti-liver fibrosis mechanism of Xiayuxue Decoction is to promote MMP-9 mRNA expression and inhibits TIMP-1 mRNA expression.