中文摘要：

为了获得羊痘病毒γ干扰素受体（interferon-γreceptor,γ-IFNR）基因,用绵羊睾丸细胞培养山羊痘病毒疫苗株G14-STV44-55,提取其基因组为模板,设计γ-IFNR基因特异性引物并进行PCR扩增,将其产物克隆至pGEM-T Easy载体,并进行了测序和序列分析。电泳结果显示,扩增出了2 600 bp左右的DNA片段;测序结果表明,成功克隆了γ-IFNR基因,其大小828 bp,基因内存在一个PmeⅠ单一酶切位点,在编码区有早期转录终止信号TTTTTN（T）T;核苷酸和氨基酸同源性分析表明,弱毒疫苗株G14-STV44-55γ-IFNR基因与基因BANK中发表的8个参考毒株的同源性在92%和89%以上,说明羊痘病毒γ-IFNR基因具有较高的保守性。

英文摘要：

The capripoxvirus live attenuated vaccine strain G14-STV44-55 was cultured with sheep testis cell line,and the genomic DNA was extracted from the virus strain,and a pair of specific primers were designed in order to amplify a interferon-γ receptor（γ-IFNR） gene.The PCR product approximately 2 600 bp in size was cloned into pGEM-T Easy vector.Sequencing of cloning confirmed that the γ-IFNR gene was obtained successfully.It is 828 bp in size,and a PmeⅠ site and a transcription termination signal TTTTTN（T） T were found in the gene.Homology analysis showed that G14-STV44-55 strain shared 92% and 89% homologies with the reference strains in levels of nucleotide and amino acid,indicating that γ-IFNR gene is very conservative.