中文摘要：

目的：研究芪玄抑甲宁对甲亢大鼠Th17细胞相关因子的调控影响。方法：将雌性SD大鼠随机分成空白组（生理盐水组）和模型组,以尾静脉注射小肠结肠炎耶尔森氏菌造模,模型组大鼠随机分为模型组、芪玄抑甲宁14.4 g/kg、7.2 g/kg、3.6 g/kg剂量组和甲巯咪唑组（3mg/kg）。用酶联免疫法检测大鼠血清中IL-17、IL-6、TGF-β水平,免疫组化法和Real-time PCR法检测甲状腺组织中IL-17、IL-17R和IL-17mRNA、IL-17RmRNA的蛋白表达。结果：芪玄抑甲宁（14.4 g/kg）可以明显降低甲亢大鼠血清中IL-17、IL-6和TGF-β的水平,回调甲亢大鼠甲状腺组织中IL-17、IL-17R和IL-17mRNA、IL-17RmRNA的蛋白表达水平。结论：芪玄抑甲宁对Th17细胞的相关因子具有调控影响,这可能是芪玄抑甲宁治疗甲亢的重要作用机制之一。

英文摘要：

Objectives：To study the regulatory effect of Qixuanyijianing on Th17 cells of rats with hyperthyroidism.Methods：60 SD female rats were divided into control group and hyperthyroidism groups.Hyperthyroidism rats were copied by injecting Yersinia enterocolitica into rats’caudal vein.The hyperthyroidism rats were randomly divided into model group,14.4 g/kg,7.2 g/kg,3.6 g/kg dose group of Qixuanyijian-ing and positive group（3mg/kg）.The levels of IL-17,IL-6 and TGF-βwere measured by ELISA.Immunohistochemistry method and the Real-time PCR were used to measure the level of IL-17,IL-17R and IL-17mRNA,IL-17RmRNA protein expression of thyroid tissues.Results：Qixuanyijianing-group（14.4 g/kg）can reduce the serum level of IL-17,IL-6,TGF-βof the hyperthyroidism rats and IL-17 in thyroid tissue,IL-17 R and IL-17 mRNA,significantly decrease the protein expression level of IL-17RmRNA,which show the regulatory effect of Qixuanyijianing to Th17 cells.Conclusions：Qixuanyijianing has an regulatory influence on Th17 cells,which may be an important mechanism of Qixuanyijianing on hyperthyroidism.