中文摘要：

完全的 cDNA 和 ribosomal proteinsS23 (AmphiS23 ) 和从文昌鱼 Branchiostoma belcher i tsingtauense 的 L35 (AmphiL35 ) 的推出的氨基酸序列在这 study.AmphiS23 cDNA 被识别长是 546 bp 并且编码 143 氨基酸的蛋白质。它有 15,851 Da 的一个预言的分子的团， 10.7 .AmphiL35 cDNA 的 pI 包括 473 bp ，并且与 14,543 Da 的一个预言的分子的团和 10.8 .AmphiS23shares 的 pI 编码 123 氨基酸的论坛蛋白质有它在脊椎动物的相当或相同事物的超过83%身份和在 invertebrates.AmphiL35 的有那些的超过84%身份是超过63%与它在脊椎动物并且多于的对应物相同52%与那些相同在无脊椎动物，DNA 印迹分析在文昌鱼 B.belcheri tsingtauense.This 的染色体表明了 S23 基因的 1-2 拷贝和 L35 基因的 2-3 拷贝的存在在到 S23 基因的 6-13 拷贝和在老鼠染色体的 L35 基因的 15-17 拷贝的存在的鲜明对比。家务基因喜欢 S23，是清楚的， L35 在脊椎动物系经历了大规模复制，增强基因 / 染色体复制假设。

英文摘要：

The complete cDNA and deduced amino acid sequences of the ribosomal proteins S23 （AmphiS23） and L35 （AmphiL35） from amphioxus Branchiostoma belcheri tsingtauense were identified in this study. AmphiS23 cDNA is 546 bp long and encodes a protein of 143 amino acids. It has a predicted molecular mass of 15,851 Da and a pI of 10.7. AmphiL35 cDNA comprises 473 bp, and codes for a protein of 123 amino acids with a predicted molecular mass of 14,543 Da and a pI of 10.8. AmphiS23 shares more than 83% identity with its homologues in the vertebrates and more than 84% identity with those in the invertebrates. AmphiL35 is more than 63% identical to its counterparts in the vertebrates and more than 52% identical to those in the invertebrates. Southern blot analysis demonstrated the existence of 1-2 copies of the S23 gene and 2-3 copies of the L35 gene in the genome of amphioxus B. belcheri tsingtauense. This is in sharp contrast to the presence of 6-13 copies of the S23 gene and 15-17 copies of the L35 gene in therat genome. It is clear that the housekeeping genes like S23 and L35 underwent a large-scale duplication in the vertebrate lineage, reinforcing the gene/genome duplication hypothesis.