中文摘要：

二全身的 cDNAs ，命名 CaM1a 和 CaM1b ，编码高度 conservedcalmodulinl ( CaM1 )蛋白质，从文昌鱼 Branchiostoma belcheritsingtauense.There 的 cDNA 图书馆被孤立仅仅是他们之间的二核苷酸差别，生产 CaMl 的氨基酸顺序的 CaMla 和 CaMlb.Comparison 之间的一氨基酸差别表明 theB.belcheri tsingtauense CaMla 与 B.floridae andB.lanceolatum ，果蝇 melanogaster 凸轮，海鞘类动物 Halocynthia roretzi CaMA 和软体动物 Aplysiacalifornica 凸轮，和 CaMlb 的 CaMl 蛋白质是相同的仅仅在一个位置不同( 138 ， Asn 到毒蛇)。种系发生的分析显示在所有三文昌鱼种的 CaMl 看起来编码常规凸轮 andCaMl 可能从 CaMl.Southern 污点的基因复制被导出建议有在 B.belcheri tsingtauense.Northern 污点的染色体并且在 situ 杂交分析 hows 的 CaMl 的二个拷贝二个 CaMl mRNA 抄本的存在与在不同成年纸巾的各种各样的表示层次和在文昌鱼 B.belcheri tsingtauense.The 进化和后生动物的凸轮 mRNA 的差异的胚胎的阶段，抄本也被讨论。

英文摘要：

Two full-length cDNAs, named CaMla and CaMlb, encoding the highly conserved calmodulinl （CAM1） proteins, were isolated from the cDNA library of amphioxus Branchiostoma belcheri tsingtauense. There are only two nucleotide differences between them, producing one amino acid difference between CaMla and CaMlb. Comparison of the amino acid sequence of CaM1 reveals that the B. belched tsingtauense CaMla is identical with CaM1 proteins of B. floridae and B. lanceolatum, Drosophila melanogaster CaM, ascidian Halocynthia roretzi CaMA and mollusk Aplysia californica CaM, and CaMlb differs only at one position （138, Asn to Asp）. The phylogenetic analysis indicates that the CaM1 in all th;ee amphioxus species appears to encode the conventional CaM and CaM2 might be derived from gene duplication of CaMI. Southern blot suggests that there are two copies of CaM1 in the genome of B. belcheri tsingtauense. Northern blot and in situ hybridization analysis shows the presence of two CaM1 mRNA transcripts with various expression levels in different adult tissues and embryonic stages in amphioxus B. belcheri tsingtauense. The evolution and diversity of metazoan CaM mRNA transcripts are also discussed.