中文摘要：

瞄准：为了调查低紧张超声(美国) ，在人的胃的癌房间和它的潜在的机制并且到导致了 apoptosis 建议一条新治疗学的途径到胃的癌。方法：人的 SGC-7901 胃的癌房间是有教养的在试管内并且在照耀以后与不同孵化时间在不同紧张为 10 min 由低紧张美国照耀。词法变化在显微镜下面被检验，尝试平底锅蓝色染色然后早 apoptotic 房间的百分比被流动血细胞计数(FCM ) 与两倍染色荧光黄异硫氰酸盐(FITC ) 检测 -Annexin V/propidium 碘化物(PI ) 。二维的电气泳动(2DE ) 被用来让蛋白质侧面和一些蛋白质在美国照耀被帮助矩阵的激光解吸附作用 / 电离 time-of-flight 团分光术(MALDI-TOF-MS ) 识别以后，不同地表示了。功能的分析被执行调查导致美国的房间 apoptosis 的机制。结果：apoptotic 房间的百分比在美国照耀以后增加了大约 10%(12.0 W/cm2， 12 h 文化) 。在照耀美国的房间的早 apoptosis 和第二等的坏死的百分比与增加的美国紧张增加了。而且， apoptotic 房间在美国照耀以后与增加的文化时间增加了并且在大约 12 h 到达了它的最大值。几新蛋白质在美国照耀以后出现了并且起来或下面调整了超过 2 次。一些热吃惊蛋白质(HSP ) 被发现与模仿房间的 apoptosis 的信号过程被联系。结论：低紧张美国能在人的胃的癌房间导致 apoptosis。导致美国的 apoptosis 与美国紧张 / 文化时间有关。导致美国的 apoptosis 可能是 caspases 依赖的并且 endoplasmic (嗯) 被触发压力的 apoptosis 可以也贡献它。Proteomic 试验性的系统在在美国照耀以后在癌房间发现蛋白质改变是有用的，帮助开发新癌症治疗。

英文摘要：

AIM： To investigate the low intensity ultrasound （US）- induced apoptosis in human gastric carcinoma cells and its potential mechanism and to suggest a new therapeutic approach to gastric carcinoma. METHODS： Human SGC-7901 gastric carcinoma cells were cultured in vitro and irradiated by low intensity US for 10 min at different intensities with different incubation times after irradiation. Morphologic changes were examined under microscope with trypan blue staining and then the percentage of early apoptotic cells was detected by flow cytometry （FCM） with double staining of fiuorescein isothiocyanate （FITC）- Annexin V/propidium iodide （PI）. Two-dimensional electrophoresis （2DE） was used to get the protein profile and some proteins differently expressed after US irradiation were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF-MS）. Functional analysis was performed to investigate the mechanism of US-induced cell apoptosis. RESULTS： The percentage of apoptotic cells increased about 10% after US irradiation （12.0 W/cm^2, 12 h culture）, The percentage of early apoptosis and secondary necrosis in the US-irradiated cells increased with the increased US intensity. Moreover, apoptotic cells increased with the increased culture time after US irradiation and reached its maximum at about 12 h.Several new proteins appeared after US irradiation and were up or down regulated more than 2 times. Some heat shock proteins （HSPs） were found to be associated with the signal process simulating the apoptosis of cells. CONCLUSION： Low intensity US could induce apoptosis in human gastric carcinoma cells. US-induced apoptosis is related to US intensity/culture time. US-induced apoptosis may be caspases-dependent and endoplasmic reticulum （ER） stress-triggered apoptosis may also contribute to it. Proteomic experimental system is useful in finding the protein alteration in carcinoma cells after US irradiation, helping to develop a new cancer ther