中文摘要：

瞄准：为了调查蛋白质，人的 hepatocarcinoma 细胞线 SMMC-7721 介绍，到分析specific 在 hepatocarcinoma 开始，生长和转移的过程丰富的表示蛋白质工作，到在诊断为早预言识别hepatocarcinoma特定的简历标记，并且为肝癌症治疗指向探索新药。方法：从人的 hepatocarcinoma 房间线 SMMC-7721 的全部的蛋白质被二维的电气泳动(2DE ) 分开。染色银的胶化被 2DE 软件图象主人 2D 精英分析。有趣的蛋白质点被基于帮助矩阵的激光解吸附作用 / 电离 time-of-flight 团分光术(MALDI-TOF-MS ) 并且数据库寻找采指纹的肽质量识别。结果：我们获得了人的 hepatocarcinoma 房间线 SMMC-7721 的蛋白质侧面。在 21 成功地识别的蛋白质之中， mitofilin， endoplasmic 蛋白质 ERp29， ubiquinol 细胞色素 C 还原酶建筑群核心蛋白质我， peroxisomal enoyl CoA hydratase， peroxiredoxin-4 和可能的 3-oxoacid CoA 转移酶 1 位先锋是在人的 hepatocarcinoma 房间或纸巾识别的六新奇蛋白质。识别热吃惊蛋白质的特定的函数详细被分析，并且结果建议这些蛋白质可能经由禁止几个癌症相关的压力或经由在 apoptotic 信号小径在多重点禁止 apoptosis 导致的细胞死亡支持肿瘤发生。另外的识别女伴和癌症相关的蛋白质也被分析。结论：基于 SMMC-7721 房间的蛋白质侧面，功能的分析建议识别女伴和癌症相关的蛋白质有他们的自己的小径贡献肿瘤发生，肿瘤生长和肝癌症的转移。而且， proteomic 分析被显示在癌症学习可行。

英文摘要：

AIM： TO investigate the protein profile of human hepatocarcinoma cell line SMMC-7721, to analyze the specific functions of abundant expressed proteins in the processes of hepatocarcinoma genesis, growth and metastasis, to identify the hepatocarcinoma-specific biomarkers for the early prediction in diagnosis, and to explore the new drug targets for liver cancer therapy. METHODS： Total proteins from human hepatocarcinoma cell line SMMC-7721 were separated by two-dimensional electrophoresis （2DE）. The silver-stained gel was analyzed by 2DE software Image Master 2D Elite. Interesting protein spots were identified by peptide mass fingerprinting based on matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry （MALDI-TOF-MS） and database searching. RESULTS： We obtained protein profile of human hepatocarcinoma cell line SMMC-7721. Among the twenty-one successfully identified proteins, mitofilin, endoplasmic reticulum protein ERp29, ubiquinol-cytochrome C reductase complex core protein I, peroxisomal enoyl CoA hydratase, peroxiredoxin-4 and probable 3-oxoacid CoA transferase 1 precursor were the six novel proteins identified in human hepatocarcinoma cells or tissues. Specific functions of the identified heat-shock proteins were analyzed in detail, and the results suggested that these proteins might promote tumorigenesis via inhibiting cell death induced by several cancer-related stresses or via inhibiting apoptosis at multiple points in the apoptotic signal pathway. Other identified chaperones and cancer-related proteins were also analyzed. CONCLUSION： Based on the protein profile of SMMC-7721 cells, functional analysis suggests that the identified chaperones and cancer-related proteins have their own pathways to contribute to the tumorigenesis, tumor growth and metastasis of liver cancer. Furthermore, proteomic analysis is indicated to be feasible in the cancer study.