中文摘要：

从增加反应器中光合细菌的细胞持有量并强化光能利用和底物传输的角度出发,构造了新型的微槽透光板式光合制氢反应器。通过沼泽红假单胞菌（Rhodopseudomonaspalustris）的连续流产氢实验研究表明：当以葡萄糖为碳源底物时,在反应器光波长为590 nm、光照强度为9 W/m2、进口底物浓度为55 mmol/L、流速为960 m L/h运行工况下,产氢速率、底物降解效率和光能转化效率均有显著增加,分别达到1.17 mmol/（L·h）、77.5%和20.15%。研究表明,选择与光合细菌产氢代谢相适应的光波长和光照强度以强化光合磷酸化过程,并通过传质强化以促进底物的传输,是提高连续流光合制氢反应器产氢性能的有效方法。

英文摘要：

Aiming for promoting cell-immobilization in the bioreactor and enhancing continuous hydrogen production, a novel groove-type flat panel photo-biological reactor（ GFPR） was developed.Photosynthetic bacteria（ PSB） strain of Rhodopseudomonaspalustris was successfully attached on the groove-type surface of transparent material and generated PSB type biofilm. Strategies on improving continuous photo-biological hydrogen production within GFPR were comprehensively investigated.Experimental results revealed that hydrogen production rate,substrate degradation efficiency and light conversion efficiency of GFPR were obviously increased to 1. 17 mmol /（ L·h）,77. 5% and 20. 15%,respectively,under specific operating conditions of 590 nm of light wavelength,9 W / m2 of light intensity,55 mmol / L of inlet substrate concentration and 960 m L / h of flow rate with glucose-based medium.Methods such as choosing proper light wavelength,light intensity to accelerate photophosphorylation for photosynthetic bacteria to accomplish hydrogen production metabolism and enhancing substrate transportation using convective mass transfer process were proved to be the effective way for promoting performance of photo-biological hydrogen production within the cell immobilized reactor operated under continuous flow mode. The experiment results were introduced to further research photo-biological reactor for practical hydrogen production.