中文摘要：

从自然界多处污泥中取样,采用选择性培养基初步富集出光合细菌混合菌群,采用菌体部分回流法对混合菌群进行筛选和优化,并研究了混合菌群以牛粪污水为原料的产氢特性。结果表明：从活性污泥中利用选择性培养基富集出的光合细菌混合菌群,生长快速、稳定,生长条件和产氢条件都比纯种细菌要求低。采用菌体部分回流装置筛选出了具有较高产氢活性的光合产氢混合菌群,菌体回流的最佳条件为：菌体回流时间36 h,菌体回流量30%,此时混合菌群的产氢活性较高,最大产氢速率达到28.3 m L/（L·h）,平均氢气体积分数为55%。混合菌群以牛粪污水为原料产氢时,产氢持续时间216 h,平均产氢速率为11.65 m L/（L·h）,原料利用率为71.48%,平均原料转化率为52.60 m L/g。

英文摘要：

For photo-hydrogen production offers the possibility of being renewable and carbon neutral, it has promising prospects in the clean energy future. The microorganisms from several natural sludge pools where has plenty of sunlight and with high organic load were used as samples. The mixed culture of photosynthetic bacteria （PSB） was cultivated by using selective medium method. Bacteria partial reflux device was employed for screening and optimization of mixed culture. The hydrogen production characteristics of mixed culture of PSB using cattle manure wastewater were also investigated. The results showed that, the mixed PSB culture grew fast and stable, and the conditions for its growth and hydrogen production were less strict than that of pure PSB. The optimum conditions for bacterial reflux were cell reflux time of 36 h and cell reflux volume of 30%. The maximum hydrogen production rate was 28.3 mL/（L-h）, and mean concentration of H2 was 55%. When using cattle manure wastewater as substrate for biohydrogen production by mixed PSB, the hydrogen production duration was up to 216 h, the average hydrogen production rate was 11.65 mL/（L.h） , substrate utilization ratio was 71.48% , and the average substrate conversion rate was 52. 60 mL/g.