采用二极管阵列(PDA)检测器,建立了超高效液相色谱(UPLC)定性定量分析土壤中16种多环芳烃(PAHs)的方法。并将该方法与传统高效液相色谱(HPLC)的分析性能进行了详细的比较。研究结果表明,采用UPLC法分析16种PAHs具有分析速度快(13.5min)、检出限低(2~20pg)、灵敏度高等优点。
An analytical method based on ultra performance liquid chromatography(UPLC)coupled with a photodiode array(PDA)detector has been developed for the simultaneous determination of 16 polycyclic aromatic hydrocarbons(PAHs).The comparison of UPLC and the conventional high performance liquid chromatography(HPLC)has been carried out.The major advantages of UPLC over HPLC include the faster analysis speed,considerably increased sensitivity and significantly improved detection limit.By UPLC,the whole analytical process can be performed in 13.5 min and the limits of detection for the 16 PAHs were between 2 pg and 20 pg.