中文摘要：

利用光谱修正技术研究脱氧核糖核酸（DNA）与5-Br-PAN-S（L）分子间的相互作用，通过pH8．04的介质中DNA与5-Br-PAN-S反应的光谱研究，测定了结合产物的物理化学参数：10℃时探针L与DNA的结合比为4．3，结合常数K=0．6118×10^6；30℃时探针L与DNA的结合比为3．7，结合常数K=0．6769×10^6。此结合反应符合Langmuir吸附方程，该方法可应用于痕量DNA的测定。

英文摘要：

The interaction of molecules of DNA with 5-Br-PAN-S was studied by applying the spectral correction technique. Through absorptio-spectrometry, In a buffer medium of pH 8. 04, the physical chemical parameters of the combination product were studied by spectro-photometry. The combination ratio between 5-Br-PAN-S and DNA （L ： DNA） at 10 ℃ was found to be 4.3, with combination constant of 0. 611 8×10^6 while at 30 ℃, the ratio was 3. 7 with combination constant of 0. 676 9 ×10^6. It was found that the interaction conformed with the Langmuir adsorption equation. This interaction was feasible to be used for the determination of DNA.