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奶牛乳房炎相关功能基因组学与蛋白质组学研究进展
  • 期刊名称:湖北农业科学, 47(5):601-605,2008
  • 时间:0
  • 分类:S823.91[农业科学—畜牧学;农业科学—畜牧兽医] Q78[生物学—分子生物学]
  • 作者机构:[1]四川农业大学动物医学院,四川雅安625014, [2]甘肃农业大学动物医学院,兰州730070, [3]中国农业科学院北京畜牧兽医研究所,北京100094
  • 相关基金:科技部国际科技合作重点项目(2005DFA30720);国家自然科学基金项目(30571329);四川农业大学青年科技创新基金项目(00231800)
  • 相关项目:奶牛乳房炎抗性相关功能蛋白研究
中文摘要:

功能基因组学与蛋白质组学是在基因组和蛋白质整体水平上分析基因功能,研究技术包括差异显示反转录PCR、表达序列标签、基因表达系列分析、生物芯片、RNA干涉、生物信息学、二维凝胶电泳(2-DE)、质谱(MS)等。综述了功能基因组学和蛋白质组学及其研究技术在奶牛乳房炎相关的基因表达、抗性候选基因鉴定、病原微生物蛋白质组学、基因治疗、新诊断靶标筛选等的应用研究进展及展望。

英文摘要:

Functional genomics and proteomics were systemically and comprehensively analyzed the function of genes at the level of the whole-genome and proteins. The main research techniques for functional genomics and proteomics include included differential display reverse transcription-PCR (DDRT-PCR), expressed sequence tag, serial analysis of gene expression, biochip, RNA interference, two-dimensional gel electophoresis (2-DE), mass spectrometry (MS) and bioinformatics. This paper reviewed current research progress on mastitis-related functional genomics and proteomics, which included mastitis-related gene expression, identification of candidate genes for mastitis resistance, proteomics of bacterial pathogens, gene therapy, treatment and diagnosis biomarkers for mastitis. Furthermore, took a view of functional genomics and proteomics prospect to study of mastitis in dairy cows.

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