中文摘要：

电子克隆是基因克隆的新策略。利用一个患乳房炎奶牛外周血白细胞中差异表达的EST序列为信息探针进行电子克隆，根据电子克隆序列设计引物，以奶牛外周血白细胞cDNA为模板进行RT—PCR验证，PCR产物克隆入pGEM—T质粒载体，测序得到的序列已被GenBank收录（DQ010409）。该基因被命名为牛锌指蛋白313（Znf313）基因，开放阅读框1-693bp，推测编码230个氨基酸，预测其编码蛋白分子量为25．67kD，等电点6．90。

英文摘要：

In silico cloning was a new strategy of gene cloning developed with the development of genome, EST projects and bio-informatics. Using one differentially expressed EST in peripheral blood leukocytes of Holstein cows with mastitis as a querying probe, a novel cDNA sequence was obtained from bovine EST database of GenBank, which was named bovine zinc finger protein 313 （Znf313） gene. The sequence of in silico cloning was verified by RT-PCR, and inserted into pGEM-T vector for sequencing. The bovine Znf313 gene cDNA contains a 693bp open reading frame, encoding 230aa protein, putative protein molecular weight is 25.67kD, isoelectric point is 6.90. The sequence has been submitted to GenBank with the accession number DQ010409.