中文摘要：

[目的]研究快速老化小鼠SAMP8和同品系正常小鼠SAMR1海马、皮质神经元中淀粉样多肽蛋白（Aβ）、亲环蛋白D（CypD）差异表达是否与其基因CDS区DNA序列单核苷酸多态性（SNP）有关,以及＂三焦针法＂对Aβ、CypD基因CDS区DNA序列是否有干预作用。[方法]选取快速老化小鼠SAMP8为模型,采用PCR扩增、SNP一代测序等法比对小鼠皮质、海马组织中Aβ基因CDS区引物设计位点（17个）以及CypD基因CDS区引物设计位点（6个）的DNA序列,并观察＂三焦针法＂对Aβ、CypD基因CDS区DNA序列是否有干预作用。[结果]SAMR1正常对照组、SAMP8对照组小鼠皮质、海马Aβ和CypD基因CDS区引物设计位点DNA序列均未发生SNP,针刺组小鼠皮质、海马Aβ和CypD基因CDS区引物设计位点DNA序列未发生变化。[结论]Aβ、CypD mRNA及蛋白差异表达与其基因CDS区DNA序列SNP无关,＂三焦针法＂对Aβ、CypD基因CDS区DNA序列无干预作用,并推测AD的发生与Aβ、CypD基因CDS区DNA序列SNP无关,＂三焦针法＂并非通过改变Aβ、CypD基因CDS区DNA序列的途径实现对Aβ、CypD mRNA及蛋白含量的调节作用。

英文摘要：

[Objective] To study the differential expression of Aβ and CypD in hippocampal and cortical neurons of senescence accelerated mice SAMP8 and the same strain normal mice SAMR1 were caused by single nucleotide polymorphism（SNP） in their gene CDs re-gions,DNA sequence,and to study if the triple energizer techniques of needling has intervention effect on DNA sequence of A β and CypD CDs regions in hippocampal and cortical neurons of senescence accelerated mice SAMP8 and the same strain normal mice SAMR1.[Methods] Senescence accelerated mice SAMP8 was selected as the model,using PCR amplification and SNP generation sequencing method to compare DNA sequences of Aβ and CypD CDs primer design sites in their cortex and hippocampus and to observe intervention effect of triple energizer techniques of needling on DNA sequence of A β and CypD CDs regions. [Results] SNP were not found in DNA sequences of CDs primer design sites of SAMR1 and SAMP8 mice ＇s cortex and hippocampus,and no changes were found in DNA sequences of CDs primer design sites of acupuncture group mice ＇s cortex and hippocampus. [Conclusion] No relationship was found between of Aβ and cypd mRNA and protein differential expression and SNP in DNA sequence of their gene CDs regions and no intervention effect of triple energizer techniques of needling on DNA sequence of Aβ and cypd gene CDs regions. Furthermore,it is speculated that there was no relationship between the occurrence of AD and SNP in DNA sequence of Aβ and cypd CDs regions and that triple energizer techniques of needling is not by changing the DNA sequence of Aβ and cypd CDs regions to regulate mRNA and protein expression levels of Aβ and cypd.