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Th17细胞分化、调节及效应研究进展
  • 期刊名称:生物化学与生物物理进展。2008, 35(2): 117 -123
  • 时间:0
  • 分类:R3[医药卫生—基础医学]
  • 作者机构:[1]Institute of Basic Medical Sciences, Beijing, China, [2]Laboratory of Cellular and Molecular Immunology, Henan University, Kaifeng, China
  • 相关基金:This work was supported by the grants from the National Key Basic Research Program of China (2007CB512406), the National Natural Science Foundation of China (30571732) and National "863" Fund Grant of China (2006AA02A254).
  • 相关项目:基于抗原呈递细胞表型变化的I型糖尿病特异性防治研究
中文摘要:

补充 C5 anaphylatoxin 受体是那发信号通过的七个跨越 transmembrane G 联合蛋白质的受体总科的一个成员官方补给并且 G16。C5aR 主要在 neutrophils,巨噬细胞和 endothelial 房间上被表示。C5a 和 C5aR 相互作用在众多的生物效果例如起一个重要作用在导致煽动性的损坏的 vivo cytokine 暴风雨里。认为人的外部血 neutrophils 和他们的短一半的收集的限制是生活,稳定地,为学习 C5aR 的生物效果的 transfected 房间线被需要。在这研究,我们 transfected C5aR 基因进 Molt-4 房间线并且检验了宫外的 C5aR 的功能。我们的结果证明在有人的 C5a 的 Molt-4 房间线和他们的相互作用的 C5aR 的稳定的表示导致了 ERK1/2 phosphorylation, Ca++ 流入。这根稳定的 transfected 房间线可以为学习与 C5a 和 C5aR 相互影响和为 C5aR 特定的抗体开发有关的信号小径提供一个有用工具。

英文摘要:

The complement C5 anaphylatoxin receptor is a member of the seven transmembrane-spanning G protein-coupled receptor superfamily that signals through Gcxi and Gtz16. C5aR is mostly expressed on neutrophils, macrophages and endothelial cells. C5a and C5aR interaction plays an important role in numerous biological effects such as in vivo cytokine storm which results in inflammatory damage. Considering the limitation of collection of human peripheral blood neutrophils and their short half life, the stably transfected cell line for studying the biological effects of C5aR is needed. In this study, we transfected C5aR gene into Molt-4 cell line and examined the function of ectopic C5aR. Our results showed stable expression of the C5aR in Molt-4 cell line and their interaction with human C5a induced ERKI/2 phosphorylation, Ca++ influx. This stable transfected cell line may provide a useful tool for studying signal pathways related to C5a and C5aR interplay and antibody development specific for C5aR. Cellular & Molecular Immunology.

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