中文摘要：

目的：建立中药淡竹叶中日本当药黄素的RP-HPLC含量测定方法。方法：高效液相色谱法,采用Cosmosil 5 C18-ms-Ⅱ色谱柱（4.6 mm×250 mm,5μm）,流动相乙腈-0.05%醋酸水溶液（14：86）,流速1.0 mL·min^-1,分析时间20 min,检测波长350 nm,进样体积10μL。结果：日本当药黄素进样量与峰面积呈良好线性关系,线性范围为0.3～5.0μg,r=1.000 0（n=5）,回收率为100.2%（n=6）。结论：该方法首次测定了8个地区淡竹叶药材中日本当药黄素的含量,该方法准确、简便、重复性好。

英文摘要：

Objective：To develop a reversed-phase HPLC method for the determination of swertiajaponin in the leaves of Lophatherum gracile.Method：Cosmosil 5 C18-ms-Ⅱ（4.6 mm × 250 mm,5 μm） column was adopted with the mobile phase of acetonitrile-0.05% acetic acid solution（14：86）;the flow rate was 1 mL·min^-1,and the detection wavelength was set at 350 nm.Result：The calibration curve was linear in the range of 0.3 ～ 5.0 μg（r =1.000 0）;the average recovery was 100.2 %.Conclusion：The content of swertiajaponin in the leaves of L.gracile from 8 different sources was determined for the first time.The developed method is simple,accurate with good reproducibility.