中文摘要：

目的观察NF-κB核转录因子在砷诱导人正常膀胱上皮细胞（SV-HUC-1）环氧化酶-2（COX-2）表达中的作用。方法在细胞培养液中加入不同浓度的NaAsO2,24 h后提取细胞总RNA和核蛋白,用RT-PCR和Westernblot方法分别分析COX-2 mRNA表达和NF-κB蛋白水平;选择COX-2高表达的NaAsO2处理组,再用NF-κB抑制剂（PDTC）处理,观察COX-2 mRNA表达水平的变化。结果 4、8μmol/L NaAsO2处理细胞后,COX-2 mRNA表达水平显著高于对照组（P〈0.05）;4μmol/L NaAsO2处理组细胞NF-κB蛋白水平也显著提高,高于对照组,而10μmol/LNaAsO2处理组细胞NF-κB蛋白水平低于对照组（P〈0.05）;同时用4μmol/L NaAsO2和PDTC共同处理细胞后,COX-2 mRNA表达水平降低,显著低于单纯用4μoml/L NaAsO2处理细胞的COX-2 mRNA表达水平（P〈0.05）。结论低剂量的砷能够诱导人正常膀胱上皮细胞COX-2 mRNA和NF-κB蛋白表达水平的增高,砷诱导的核转录因子NF-κB活化在砷诱导的COX-2表达水平增高中起一定作用。

英文摘要：

Objective To explore the effects of NF-κB on COX-2 mRNA expression in SV-HUC-1 cells exposed to arsenite.Methods Cells were cultured in medium contained 0,1,2,4,8 and 10 μmol/L arsenite or PDTC,the inhibitor of NF-κB for 24 h,then the expressions of COX-2 mRNA and nuclear protein NF-κB were determined by RT-PCR and Western blot,respectively.Results Compared with those in control group,the expressions of COX-2 mRNA in cells exposed to 4 and 8 μmol/L arsenite and the expressions of NF-κB in cells exposed to 4 μmol/L arsenite were increased significantly（P〈0.05）,while expressions of NF-κB nuclear proteinum in the cells exposed to 10 μmol/L arsenite were decreased significantly.On the other hand,expressions of COX-2 mRNA in cells exposed to both 4 μmol/L arsenite and PDTC were lower significantly than those in the cells only exposed to 4 μmol/L arsenite（P〈0.05）.Conclusion Findings from this study suggested that low dose of arsenic could induce the expression of COX-2 mRNA and NF-κB in SV-HUC-1 cells,while the activation of NF-κB induced by arsenic might play some role in the process mentioned above.