中文摘要：

为了建立柿属植物目标区域扩增多态性（TRAP）标记技术体系,对影响TRAP-PCR的Mg^2＋、dNTPs、Taq DNA聚合酶、固定引物和随机引物浓度比5个因素进行了优化。确定优化的反应体系为：模板DNA 40 ng,buffer 1×,Mg^2＋1.4 mmol/L,dNTPs 0.2 mmol/L,固定引物和随机引物浓度比为11∶1,Taq酶0.5 U,反应总体积为15μL。利用柿属植物EST数据库信息设计锚定引物10条,与11条随机引物组合共110对引物。利用这些引物对柿属植物6个基因型进行TRAP-PCR扩增,其中84个引物组合能扩增出清晰条带,占引物总量的76.36%;36个引物组合表现出良好的多态性扩增,并在20份柿属植物中进行了引物验证。

英文摘要：

The concentrations of Mg^2 ＋,dNTPs,Taq DNA polymerase and primers which affected TRAP（ target region amplified polymorphism）- PCR reaction were optimized for the establishment of TRAP molecular marker system in Diospyros spp. The optimum reaction system was acquired as follows： template DNA 40 ng,buffer 1 ×,Mg^2 ＋1. 4 mmol /L,dNTPs 0. 2 mmol /L,fixed primer- arbitrary primer concentration ratio 11∶ 1,Taq DNA polymerase 0. 5 U,and total volume of reaction system 15 μL. Eighty- four TRAP primer combinations which showed robust PCR amplification in 6 persimmon genotypes were screened out from 110 primer combinations（ 10 fixed primers times 11 arbitrary primers）. Thirty- six polymorphic primer combinations were verified in 20 persimmon genotypes.