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Transformation of Two VP1 Genes of O-and Asia 1-Type Foot-and-Mouth Disease Virus into Maize
  • ISSN号:1671-3990
  • 期刊名称:《中国生态农业学报》
  • 时间:0
  • 分类:S855.3[农业科学—临床兽医学;农业科学—兽医学;农业科学—畜牧兽医] S852.65[农业科学—基础兽医学;农业科学—兽医学;农业科学—畜牧兽医]
  • 作者机构:[1]Maize Research Institute, Sichuan Agricultural University, Ya 'an 625014, P.R. China, [2]Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, IER.China
  • 相关基金:Acknowledgements This work was supported by the National Natural Science Foundation of China (30800687 and 31071434), the Applied Basie Research Program of Sichuan Provincial Science and Technology Department, China (2008JY0096), the Foundation for Young Scientists of Sichuan Provincial Education Department, China (09ZB051), the Youth Innovation Project of Sichuan Agricultural University, China, and the Postdoctoral Project of Sichuan Agricultural University, China. We are grateful to Prof. Li Pan, Lanzhou Veterinary Research Institute, China, for helpful discussions in the vector construction and plant transformation experiments.
中文摘要:

在转基因的植物的抗原的表达式逐渐地为试验性的疫苗的开发被用作古典方法论的一种选择。这份报纸这里与 foot-and-mouth 疾病病毒(FMDV ) 的结构的蛋白质 VP1 的二 serotypes 在转基因的玉米为 foot-and-mouth 疾病(FMD ) 报导一个新奇口头的免疫系统的发展 viz, O- 和亚洲 1 类型分别地。转基因的小植物被聚合酶链反应(PCR ) 识别并且调查,南部的污点,和即时 PCR。而且,在转基因的植物的 VP1 基因能通过 PCR 稳定地被传给下一代,这被发现察觉。到我们的知识,这是在一次尝试的第一份报告由喂 FMDV 的二 serotypes 抗原蛋白质一起在表示了的转基因的植物在动物导致保护的全身的抗体回答。实验的结果提供把基于植物的疫苗用作饲料或饲料添加剂的可能性。

英文摘要:

The expression of antigens in transgenic plants has increasingly been used as an alternative to the classical methodologies for the development of experimental vaccines.This paper reports here the development of a novel oral immunization system for foot-and-mouth disease (FMD) in transgenic maize with two serotypes of the structural protein VP1 of the foot-and-mouth disease virus (FMDV) viz.,O-and Asia 1-type,respectively.The transgenic plantlets were identified and investigated by polymerase chain reaction (PCR),Southern blot,and real-time PCR.Moreover,it was found that the VP1 genes in transgenic plants could be transmitted stably to the next generation through PCR detection.To our knowledge,this is the first report in an attempt to induce a protective systemic antibody response in animals by feeding the transgenic plants in which two serotypes antigen protein of FMDV expressed together.Results of the experiment provide the possibility of using plant-based vaccines as feedstuff or feedstuff additives.

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期刊信息
  • 《中国生态农业学报》
  • 中国科技核心期刊
  • 主管单位:中国科学院
  • 主办单位:中国科学院遗传与发育生物学研究所 中国生态经济学会
  • 主编:刘昌明
  • 地址:河北省石家庄市槐中路286号
  • 邮编:050022
  • 邮箱:editor@sjziam.ac.cn
  • 电话:0311-85818007
  • 国际标准刊号:ISSN:1671-3990
  • 国内统一刊号:ISSN:13-1315/S
  • 邮发代号:82-973
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  • 被引量:29638