中文摘要：

目的：探讨降钙素基因相关肽（calcitonin-gene-relatedpe ptide，CGRP）对人支气管上皮细胞分泌基质金属蛋白酶-9（matrix metalloproteinase-9，MMP-9）／明胶酶B的影响及其机制。方法：采用逆转录聚合酶链式反应及明胶酶谱法，观察给予不同浓度的CGRP（10^-10-10^-6mol／L）刺激及CGRP10^-6mol／L刺激后不同时间点（6，12，18，24，36和48h）MMP-9活性和基因表达量的变化。结果：体外培养的人支气管上皮细胞能分泌MMP-9；CGRP干预呈剂量及时间依赖方式上调人支气管上皮细胞MMP-9的活性和MMP-9mRNA的表达（P〈0．01）；CGRP上调人支气管上皮细胞MMP-9活性和表达的作用可为蛋白激酶C阻断剂H-7及钙调蛋白阻断剂W-7部分逆转（P〈0．05）。结论：CGRP可明显上调人支气管上皮细胞MMP-9的活性和表达，其胞内信号转导机制与蛋白激酶和钙调蛋白信号途径有关。

英文摘要：

Objective To examine the expression of matrix metalloproteinase-9 （ MMP-9 ） in human bronchial epithelial cells treated with calcitonin-gene-related peptide （CGRP）. Methods RT-PCR and gelatin zymography were performed to examine the dynamic expression and activity of MMP-9 in human bronchial epithelial cells at different doses （10^-10, 10^-9, 10^-8, 10^-7, and 10^-6 mol/L） and different time points （ 6,12,18,24,36, and 48 h） after the stimulation of CGRP. Results The unstimulated human bronchial epithelial cells only secreted a small amount of MMP-9. After the CGRP stimulation, the expression of MMP-9 presented in a concentration-dependent （10^-l0, 10^-9, 10^-8, 10^-7, and 10^-6mol/L） and time-dependent （6,12,18,24,36, and 48 h ） manners （ P 〈 0.01 ） in human bronchial epithelial cells. The effect of CGRP could be dimin- ished by H-7 and W-7, an antagonist of protein kinase C （PKC） and calmodulin （CAM） （P 〈 0.05 ）. Conclusion CGRP can stimulate the secretion and expression of MMP-9 in human bronchial epithelial cells, and the signal transduction is partly via the PKC and CaM pathway.