中文摘要：

目的：观察人支气管上皮细胞（human bronchial epithelial cells，HBECs）上皮钙粘素（E—cadherin，E-cd）的表达，探讨E—cd在维持气道损伤修复过程中的变化及意义。方法：分别采用免疫细胞化学染色和RT—PCR的方法检测HBECs E—cd及HBECs E—cd mRNA的表达。结果：臭氧（ozone，O3）应激可使HBECs膜上E—cd的表达减少，胞浆内E—cd表达增加（P〈0．05）。随着O3应激停止后的时间延长，膜上E—cd表达逐渐增加，胞浆内E-cd表达则减少。在停止O3应激后的第32h E—cd的表达恢复正常。O3应激对HBECs E—cd mRNA的表达无明显影响（P〉0．05）。结论：HBECs有E—cd表达，O3应激影响HBECs E—cd的分布。

英文摘要：

AIM： To explore the expression of Eeadherin （E-ed） in the human bronchial epithelial cells （HBECs） and their role in the damage and injury of the airway. METHODS： The E-cd protein expression and the E-cd mRNA of HBECs were determined by immunocytochemistry analysisand RT-PTR analysis, respectively. RESULTS： Ozone-stress caused a decrease in the HBECs membrane E-cd expression and an increase the cytoplasmic E-cd expression （ P 〈 0.05）. These changes returned to normal 32 h after the ozone-stress was withdrawn. O3-stress had little effect on the expression of HBECs E-cd mRNA （ P 〉 0.05）. CONCLUSION： E- cd is distributed on HBECs. O3-stress decreases the membrane E-cd expression but increases the eytoplamie E-cd expression. The change of E-cd expression may mediate HBECs injuries induced by O3-stress.