中文摘要：

采用梯度逆流色谱技术快速分离纯化雪菊中的3,5-二咖啡酰奎宁酸和奥卡宁,以石油醚-乙酸乙酯-甲醇-水（2∶8∶2∶8,2∶8∶4∶6;v/v）为溶剂系统,上相为固定相,下相为流动相,转速800r/min,流速5.0mL/min,检测波长254nm。所得馏分通过电喷雾电离（ESI）质谱和核磁共振谱（NMR）鉴定化合物的结构。从200mg粗提物中一次分离得到两个主要化合物：3,5-二咖啡酰奎宁酸（11.2mg）和奥卡宁（10.7mg）,且3,5-二咖啡酰奎宁酸为首次从雪菊中分离得到;经高效液相色谱法分析,其纯度分别为92.82%和97.55%。研究结果表明,应用梯度逆流色谱分离雪菊中的生物活性成分快速高效。

英文摘要：

The extraction conditions were as follows： solvent system： petroleum ether-ethyl acetate- methanol-water （2： 8： 2： 8,2： 8： 4： 6; v/v）, rotation speed： 800 r/min , flow speed：5.0 mL/min, detection wavelength： 254 nm. The obtained fractions were identified by electrospray ionization mass spectrometry （ESI-MS）, 1H-nuclear magnetic resonanee（NMR） and ^13C-NMR. Two main compounds, 3, 5- dicaffeoyl quinic acid （11.2 mg） and okanin （10.7 rag） were separated from 200 mg crude extract, whose purities were 92.82% and 97.55% and analyzed by HPLC. And 3, 5-dicaffeoyl quinic acid was separated from Coreopsis tinctoria firstly. Experimental results indicate that the method is rapid and highly efficient.