中文摘要：

目的建立快速准确测定小鼠脑组织中卵磷脂（PC）和溶血卵磷脂（LPC）含量的简便方法。方法小鼠脑组织经氯仿和甲醇抽提总脂后,以氯仿/甲醇/乙酸/丙酮/水（40/25/7/4/2,v/v）为展层剂,采用单相薄层层析（TLC）并结合钼蓝定磷法测定两种磷脂的含量。结果该法测定PC和LPC的回收率分别为94.91%±6.74%和104.00%±5.38%,相对标准偏差分别为5.05%和7.10%,最低检测限为4.80nmol。用此法测得小鼠脑组织中PC和LPC的含量分别为20257.14±1022.88nmol/g脑重、420.91±29.87nmol/g脑重。结论TLC法用于小鼠脑组织中LPC等磷脂的测定,简便易操作、重现性好,不仅能够分离测定PC和LPC,而且能够分离测定小鼠脑组织中的另外其它5种磷脂。

英文摘要：

Objective To develop a method for determining the contents of phosphatidylcholine （PC） and lysophosphatidylcholine （LPC） in mouse brain. Methods The total lipids of mouse brain were extracted with Chloroform and methanol. PC and LPC were separated by one-dimensional thin-layer chromatography （TLC） using chloroform/methanol/acetic acid/acetone/water （40： 25：7：4：2, v/v/v/v） as solvent and their contents were measured by determination of phosphorus using phosphomolybdenum blue method. Results The recovery for PC and LPC was 94.91% ± 6.74% and 104.00% ± 5.38% , respectively, with relative standard deviation of 5.05 % and 7.1.0%, respectively. The limit of quantitation was 4.80 nmoles. The contents of PC and LPC in whole mouse brain assayed by this method were 20257.14 ± 1022.88 nmoles/g wet brain and 420.91 ± 29.87 nmoles/g wet brain, respectively. Conclusion The procedure described in the present study is simple, easy to deal with, and reproducible. Not only PC and LPC but also other five phospholipids in mouse brain can be assayed by this method.